中国组织工程研究2012，Vol.16Issue(1)：116-120,5.DOI:10.3969/j.issn.1673-8225.2012.01.025
幼年大鼠脑室下区N-甲基-D-天冬氨酸受体亚单位2B的表达及其细胞类型
Expression of N-methyl-D-aspartic acid receptor 2B in the subventricular zone of juvenile rats and its cell types
摘要
Abstract
BACKGROUND: N-methy-D-aspartic acid receptor (NR)2B can express on neural stem cells. In-depth study of its expression in subventricular zone (SVZ) of juvenile rats can help to reveal the mech anisms of neural stem cell proliferation, differentiation, OBJECTIVE: To detect the expression of NR2B in SVZ of juvenile rats and cellular localization of NR2B on the peak of METHODS: The 7,14, 21. 28 days postnatal SD rats were used to made 5 μm frozen sections of brain tissue. The expression of NR2B in SVZ and the cellular localization of NR2B at 14 days after birth were observed.RESULTS AND CONCLUSION: I mmunohisto chemistry s howed that there were differential expressions of NR2B in SVZof7,14, 21,28 days postnatal rats and the expression peak of NR2Bwas on 14 day; The expression of NR2B in the lateral angle of lateral ventricle was higher than that of other parts; NR2B was co- localized with glial fib Hilary acidic protein (GFAR), Nestin, βⅢtubulin,was changed following the different postnatal time. The result of double immun ofluore staining implied that NR2B might play animportant role in neurogenesis.关键词
脑室下区/N-甲基-D-天冬氨酸受体亚单位/幼年大鼠/神经干细胞/胶质纤维酸性纤维蛋白/巢蛋白/β-Ⅲ微管蛋白分类
医药卫生引用本文复制引用
张伟,高俊英,许晶,崔桂云..幼年大鼠脑室下区N-甲基-D-天冬氨酸受体亚单位2B的表达及其细胞类型[J].中国组织工程研究,2012,16(1):116-120,5.基金项目
江苏省自然科学基金(BK2009087) （BK2009087）
江苏省高校自然科学基金(07KJB310119). （07KJB310119）
