中国组织工程研究2012，Vol.16Issue(2)：201-205,5.DOI:10.3969/j.issn.1673-8225.2012.02.002
人工虎骨粉干预大鼠成骨细胞增殖和Ⅰ型胶原的表达
Effect of bionic tiger-bone powder on rat osteoblasts proliferation and typeⅠcollagen expression
摘要
Abstract
BACKGROUND: Bionic tiger-bone powder is mainly used for the treatment of osteoporosis, fracture, rheumatoid arthritis and other bone diseases, and has achieved better clinical efficacy. However, the mechanisms of osteogenesis has not clear yet. OBJECTIVE: To observe the effects of bionic tiger-bone powder on the proliferation of rat osteoblasts and the expression of typeⅠcollagen. METHODS: We cultivate primary osteoblasts with secondary enzyme digestion of the newborn rat skull bone tissue. The Concentrations of culture medium in bionic tiger-bone powder intervention group were 10-4, 10-5 and 10-6 g / L. The control group was injected with the serum-free DMEM culture medium without bionic tiger-bone powder. RESULTS AND CONCLUSION: MTT colorimetric assay results showed that bionic tiger-bone powder could improve osteoblasts proliferation vitality apparently (P < 0.05). Meanwhile the proportion of S phase and M/G2 phase cells was increased but that of G0/G1 phase cells was decreased through flow cytometry. The RT-PCR and ELISA test showed that bionic tiger-bone powder could increase the expression of osteoblasts typeⅠcollagen (P < 0.05) when handling with them in the concentration of 10-6, 10-5 and 10-4 mg/ml for 48 hours. Bionic tiger-bone powder could promote the proliferation of rat primary osteoblasts in vitro, and could promote the synthesis and secretion of typeⅠcollagen.关键词
人工虎骨粉/成骨细胞/Ⅰ型胶原/细胞增殖/大鼠分类
医药卫生引用本文复制引用
韩娜,王天兵,寇玉辉,张殿英,张培训,殷晓峰,姜保国..人工虎骨粉干预大鼠成骨细胞增殖和Ⅰ型胶原的表达[J].中国组织工程研究,2012,16(2):201-205,5.基金项目
国家自然科学基金(30973066、31040043),首都医学发展科研基金(2007-2011),北京市自然科学基金 (7113170),教育部博士点新教师基金(20090001120096) 和北京大学人民医院研究与发展基金(RDB2010-30)资助. （30973066、31040043）
