中国组织工程研究2012，Vol.16Issue(2)：223-226,4.DOI:10.3969/j.issn.1673-8225.2012.02.007
胰岛素样生长因子1及血小板源性生长因子对人退变髓核细胞生物学活性的影响
Effect of insulin-like growth factor-1 and platelet-derived growth factor on the biological potential of human degenerative nucleus pulposus cells
林旺 1刘寿坤 1王盈盈 1郭卫中 1林成寿 1王旭 1苏郁1
作者信息
- 1. 福建医科大学附属宁德市闽东医院,福建省福安市 355002
- 折叠
摘要
Abstract
BACKGROUND: Previous studies showed that insulin-like growth factor-1 (IGF-1) and platelet-derived growth factor (PDGF) can suppress apoptosis of human intervertebral cells. OBJECTIVE: To observe the effects of IGF-1 and PDGF on the biological potential of the human degenerative NP cells in vitro. METHODS: Human degenerative nucleus pulposus cells were cultured in monolayer in vitro and identified by immunohistochemical staining. The cells of passage 3 were treated separately with different growth factors and then randomly divided into 4 groups: IGF-1 group, PDGF group, IGF-1+PDGF group and control group. RESULTS AND CONCLUSION: IGF-1 and PDGF could stimulate cell proliferation and synthesis of collagen type and Ⅱaggrecan, and IGF-1 raised more content of collagen type thanⅡ the PDGF (P < 0.05), but the PDGF raised more content of aggrecan than the IGF-1 (P < 0.05). IGF-1 stimulated the synthesis of collagen typeⅠ, then PDGF inhibited the synthesis. Both the IGF-1 and PDGF can stimulate cell proliferation, the synthesis of collagen type and aggrecan, thereby enhance the Ⅱbiological potential of the human degenerative nucleus pulposus cells.关键词
胰岛素样生长因子1/血小板源性生长因子/髓核细胞/退变/组织工程分类
医药卫生引用本文复制引用
林旺,刘寿坤,王盈盈,郭卫中,林成寿,王旭,苏郁..胰岛素样生长因子1及血小板源性生长因子对人退变髓核细胞生物学活性的影响[J].中国组织工程研究,2012,16(2):223-226,4.
