中国组织工程研究2012，Vol.16Issue(2)：235-238,4.DOI:10.3969/j.issn.1673-8225.2012.02.010
丝裂霉素C影响增生性瘢痕成纤维细胞的凋亡
Effects of mitomycin C on apoptosis of hypertrophic scar fibroblasts
吴晓明 1孙奎 1张宏霞 1孙喜平 1耿琪瑛 1李树松1
作者信息
- 1. 承德医学院附属医院烧伤整形外科,河北省承德市,067000
- 折叠
摘要
Abstract
BACKGROUND: Mitomycin C has been gradually used in hyperplastic scar therapy. But the molecular mechanism underlying the effects of mitomycin C on hyperplastic scar via cell apoptosis is reported few. OBJECTIVE: To explore the effects of mitomycin C on fibroblasts apoptosis in hypertrophic scar. METHODS: Hypertrophic scar fibroblasts were cultured in vitro with five different concentrations of mitomycin C (2.5, 12.5, 50, 100, 200 mg/L). Cell cycle distribution and apoptosis of fibroblasts were detected by Annexin V-PI, and the protein expression levels of Bax and Bcl-2 in fibroblasts were detected with Western blotting. RESULTS AND CONCLUSION: Mitomycin C blocked the growth of hyperplastic scar fibroblasts in G0/G1 period, and induced apoptosis of hyperplastic scar fibroblasts in an obvious concentration-dependent manner. Bax protein expression levels increased and Bcl-2 protein expression levels decreased in hyperplastic scar fibroblasts after treated with 2.5-200 mg/L mitomycin C for 24 hours (P < 0.05). These findings indicate that mitomycin C has the possibility to promote fibroblasts apoptosis through increasing Bax expression and decreasing Bcl-2 expression in hyperplastic scar fibroblasts.关键词
丝裂霉素C/成纤维细胞/增生性瘢痕/Bcl-2/Bax/细胞凋亡分类
医药卫生引用本文复制引用
吴晓明,孙奎,张宏霞,孙喜平,耿琪瑛,李树松..丝裂霉素C影响增生性瘢痕成纤维细胞的凋亡[J].中国组织工程研究,2012,16(2):235-238,4.
