中国组织工程研究2012，Vol.16Issue(2)：282-286,5.DOI:10.3969/j.issn.1673-8225.2012.02.021
小鼠卵巢颗粒细胞适宜培养体系及激素干预的影响
Suitable culture system of mouse ovarian granulosa cells and effect of hormone intervention
摘要
Abstract
BACKGROUND: The in vitro growth characteristics and basic biological characteristics of granulosa cells can be understood by building a stable and high-efficiency in vitro culture system of granulosa cells. OBJECTIVE: To build and optimize a primary culture system of mice ovarian granulosa cells, and to explore the effects of pregnant mare serum gonadotropin, human chorionic gonadotropin on the cell proliferation and cytoskeleton. METHODS: Mouse granulosa cells were separated and cultured in the culture systems of RPMI1640, HTF and BIO-AMF2, respectively. Pregnant mare serum gonadotropin and human chorionic gonadotropin were added to the culture systems of granulose cells in generation 3. RESULTS AND CONCLUSION: BIO-AMF2 culture system was better than RPMI1640 and HTF culture systems. Cells converged together and represented a cobblestone-like appearance on the 4th day after primary culture. The granulosa cells showed a positive reaction to C-kit immunohistochemical staining. According to cell cycle determination, most of the in vitro cultured granulosa cells were at G1 stage. Hormone showed a promoting effect on the proliferation of granulosa cells on the 2nd day after cultivation. This promoting effect was the most obvious on the 4th day after cultivation. But no significant effects of hormone on the granulosa cell morphology and cytoskeleton were detected.关键词
卵巢/颗粒细胞/体外培养/激素/细胞骨架分类
医药卫生引用本文复制引用
陈毅斐,许程洁,王鑫泉,徐营..小鼠卵巢颗粒细胞适宜培养体系及激素干预的影响[J].中国组织工程研究,2012,16(2):282-286,5.基金项目
浙江省自然科学基金(Y2110873)、嘉兴市科技局科研重点项目(2007A22013)、嘉兴市科技局科研项目(2010AY1098)、浙江省大学生科技创新活动计划项目(2010R417004). （Y2110873）
