中国组织工程研究2012，Vol.16Issue(2)：295-298,4.DOI:10.3969/j.issn.1673-8225.2012.02.024
高压氧前处理脊髓损伤大鼠前角运动神经元的凋亡
Apoptosis of anterior horn motor neurons after hyperbaric oxygen preconditioning in spinal cord injury rats
摘要
Abstract
BACKGROUND: Spinal cord injury is difficult to repair. The protection of relict neurons is the key for promoting neural regeneration. OBJECTIVE: To test whether spinal cord anterior horn motor neurons are protected by hyperbaric oxygen preconditioning through inhibition of early apoptosis. METHODS: A total of 26 Wistar male rats were randomly divided into two groups: control group and hyperbaric oxygen group. Rats in hyperbaric oxygen group were administrated with hyperbaric oxygen for 5 days. Complete spinal cord (T9-T10) transection model was constructed on the 5th day after hyperbaric oxygen. In the meantime, complete spinal cord (T9-T10) transection model was constructed for control rats. RESULTS AND CONCLUSION: Nissl staining demonstrated that hyperchromic cells were common in the two groups at 8 hour and 1 day after spinal cord transection at T9–T10. The number of hyperchromic cells in the hyperbaric oxygen group was less than that in the control group. TUNEL staining demonstrated that the massive apoptotic neurons were found at 8 hour and 1 day after the spinal cord transection at T9–T10 in rat anterior horn of spinal cord in both groups; the number of apoptotic neurons decreased on the 3rd day. The number of apoptotic spinal cord anterior horn motor neurons in the hyperbaric oxygen group was smaller than that in the control group at 8 hour and 1 day after hyperbaric oxygen (P < 0.05, P < 0.01). These findings indicate that hyperbaric oxygen preconditioning has a protective effect on anterior horn motor neurons after spinal cord injury.关键词
高压氧/预处理/脊髓损伤/前角运动神经元/凋亡/大鼠分类
医药卫生引用本文复制引用
李洪鹏,巴方,白丹,高杰..高压氧前处理脊髓损伤大鼠前角运动神经元的凋亡[J].中国组织工程研究,2012,16(2):295-298,4.基金项目
国家自然科学基金资助项目(30872669)资助. （30872669）
