中国组织工程研究2012，Vol.16Issue(3)：391-395,5.DOI:10.3969/j.issn.1673-8225.2012.03.002
热致相分离法制备聚羟基丁酸酯-羟基戊酸酯纳米纤维支架及其表征
Preparation and characterization of poly(hydroxybutyrate-co-hydroxyvalerate) nanofiber scaffolds using thermally induced phase separation method
摘要
Abstract
BACKGROUND: Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) is a polymer of microbial source with excellent biocompatibility and processing performance, which has great potential to be used as a biological material. OBJECTIVE: To investigate the synthesis methods and crystallization of nanofibrous matrix of a semicrystalline biopolyester PHBV via thermally induced phase separation. METHODS: The formation of the matrix was characterized by scanning electron microscopy, wide angle X-ray diffraction, Fourier transform infrared and differential scanning calorimetry. RESULTS AND CONCLUSION: The quenching/gelation temperatures showed a great effect on the crystallization and thermal property of the nanofibers, then affected the morphology of the scaffolds. The crystallization of the PHBV matrix during the phase separation process primarily involved HB units with small amounts of crystals incorporating HV units at higher gelation temperatures. When the quenching/gelation temperature decreased, the crystallinity and crystallite size were decreased but better crystalline perfection was obtained by excluding HV units from the PHB lattice. Meanwhile, cells experimental results show that nanofibers structure of scaffolds are really more conducive to cell adhesion and growth. The morphological and structural dependence on temperature may provide a simple way to fine tune the properties of PHBV scaffolds including biodegradability and bioresponsiveness to cellular activity.关键词
聚羟基丁酸酯-羟基戊酸酯/纳米纤维/基质/支架/生物相容性分类
医药卫生引用本文复制引用
陈聪,王迎军,邵俊东,杜昶..热致相分离法制备聚羟基丁酸酯-羟基戊酸酯纳米纤维支架及其表征[J].中国组织工程研究,2012,16(3):391-395,5.基金项目
长江学者和创新团队发展计划资助(IRT0919),课题名称:新型仿生功能化硬组织修复材料.华南理工大学中央高校基本科研业务经费(2009zz0004),课题名称:硬组织生物矿化机理与仿生型医用材料研究.新世纪优秀人才支持计划资助(NCET-08-0210). （IRT0919）
