中国组织工程研究2012，Vol.16Issue(3)：471-474,4.DOI:10.3969/j.issn.1673-8225.2012.03.020
聚乳酸-羟基乙酸共聚物/Ⅰ型胶原/壳聚糖人工硬脊膜在兔脊髓损伤模型中的应用
Effects of poly(lactic-co-glycolic acid)/type-Ⅰcollagen/chitosan composite membrane in rabbit models of spinal cord injury
摘要
Abstract
BACKGROUND: Autologous tissue, allograft tissue, and dural substitute materials originated from animals are all difficult to reduce the morbidity and mortality of spinal cord injury. OBJECTIVE: To study the effects of poly(lactic-co-glycolic acid) (PLGA) /type-Ⅰcollagen/chitosan composite membrane in rabbit models with spinal cord injury. METHODS: Seventy rabbits were randomly divided randomly into sham-operation group (n=10, simple laminectomy without spinal cord injury), model group (n=20, spinal cord injury without treatment), chitosan group (n=20, spinal cord injury treated with artificial dura mater of chitosan), and composite group (n=20, spinal cord injury treated with PLGA/type-Ⅰcollagen/chitosan composite membrane). RESULTS AND CONCLUSION: Twenty-four hours after spinal cord injury, the motor behavior scores was higher in the chitosan group and composite group than model group (P < 0.01), and the scores in the composite group were also higher than those in the chitosan group (P < 0.05). The latency of somatosensory evoked potential was longer in the model, chitosan, and composite groups than the sham-operation group after spinal cord injury. The latencies were increased at 6 hours after spinal cord injury and reached the peak at 24 hours, and then began to decrease. Two days after spinal cord injury, the differences in the latencies were insignificant among the model, chitosan and composite groups. The apoptotic rate of the sham-operation group was lower than that of other groups (P < 0.05). At 6 and 24 hours after spinal cord injury, the apoptotic rates of the chitosan and composite groups were lower than that of the model group (P < 0.05). Early surgical intervention with PLGA/type-Ⅰcollagen/chitosan composite membrane can acquire best neurological functional recovery in rabbit models of spinal cord injury.关键词
聚乳酸-聚乙醇酸共聚物/壳聚糖/Ⅰ型胶原/人工硬脊膜/脊髓损伤分类
医药卫生引用本文复制引用
白万山,王新伟,袁文,王占超,梁磊,王会学..聚乳酸-羟基乙酸共聚物/Ⅰ型胶原/壳聚糖人工硬脊膜在兔脊髓损伤模型中的应用[J].中国组织工程研究,2012,16(3):471-474,4.基金项目
上海市科学技术委员会科研计划项目课题(08411963800),课题名称:人工硬脊膜非缝合技术修复硬脊膜损伤的相关研究. （08411963800）
