中国组织工程研究2012，Vol.16Issue(4)：695-698,4.DOI:10.3969/j.issn.1673-8225.2012.01.029
创伤性关节炎软骨中基质金属蛋白酶13及组织特异性抑制剂1的表达
Expression of matrix metalloproteinase 13 and tissue inhibitor of metalloproteinase 1 in cartilage of traumatic osteoarthritis models
摘要
Abstract
BACKGROUND: The mechanism of articular cartilage degeneration in traumatic osteoarthritis is not yet clear. In recent years,studies found that imbalance of synthesis and degradation of extracellular matrix is one of the important reasons for the cartilage degeneration. Matrix metalloproteinase (MMPs) may plays a decisive role in this process.OBJECTIVE: To investigate the expression of MMPs-13 and tissue inhibitor of metalloproteinase -1 (TIMP-1) in cartilage of traumatic arthritis model, and relationship between MMPs-13, TIMP-1 and cartilage degeneration.METHODS: Healthy adult New Zealand rabbits were used to construct mild and severe traumatic arthritis models which were established with gravity method (1.33 kg, 46 cm height and 0.43 kg, 20 cm height, respectively).RESULTS AND CONCLUSION: Hematoxylin-eosin staining and immunohistochemical staining showed that the expression of both MMP-13 and TIMP-1 were significantly increased in the mild and severe traumatic groups (P < 0.05). Expression of TIMP-1 in the severe traumatic group was higher than that of in the mild traumatic group (P < 0.05). This shows that there is certain increase of MMP-13 and TIMP-1 in post -traumatic osteoarthritis. MMP-13 and TIMP-1 together to promote the degeneration of articular cartilage, and the over-expression of TIMP-1 may be one of the reasons in the further impairment to articular cartilage.关键词
组织特异性抑制剂1/创伤性关节炎/软骨/基质金属蛋白酶13/软组织构建分类
医药卫生引用本文复制引用
李强,唐际存,王锐英,贝朝涌,辛林伟,茹嘉,肖荣驰..创伤性关节炎软骨中基质金属蛋白酶13及组织特异性抑制剂1的表达[J].中国组织工程研究,2012,16(4):695-698,4.基金项目
桂林市科学研究与技术开发计划资助项目.项目名称:卫生安全与健康关键技术研究开发.课题名称:强力霉素对退变腰椎间盘中MMP-3表达水平的调控.合同编号:20070213-1. （）
