中国组织工程研究2012，Vol.16Issue(5)：809-812,4.DOI:10.3969/j.issn.1673-8225.2012.05.012
新西兰雌-雄兔皮肤移植模型体内细胞毒性实验
Cytotoxicity assay of New Zealand female-male rabbit skin graft models in vivo
摘要
Abstract
BACKGROUND:The establishment of an effective assessment model for monitoring post-transplant recipient's immune status is the difficulty in current researches.OBJECTIVE: To establish an in vivo method of cytotoxicity assay in rabbits; to verify whether the in vivo cytotoxicity assay candetect the immune status of the recipient rabbits.METHODS: New Zealand female-male rabbit skin graft models were established; the untransplanted female and male rabbitsserved as control. The spleens of both female and male rabbits were removed to prepare single cell suspension; and then live celldye hydroxyfluorescein diacetate succinimidyl ester of different concentrations were added to stain and prepare for the 1:1 mixedcell suspension. Peripheral blood samples of the male rabbits were collected at 1, 2, 3 and 8 hours after the infusion of the mixedcell suspension. Flow cytometry was used to detect the ratio of the two kinds of fluorescytes in the peripheral blood; the killingrate of specific cells in the donors was calculated.RESULTS AND CONCLUSION: The female-male rabbit model of skin graft rejection was established in the 2nd week after skintransplantaion. The ratio changes in the peripheral blood of model group and control group were different after the infusion ofmixed cell suspension; the killing rate in the male rabbits of the model group was significantly higher than that of the control groupat 1, 2, 4 and 8 hours after infusion (P < 0.01). These findings indicate that cytotoxicity assay in vivo can be used to monitorimmune state; it can directly reflect the specific immune environment in vivo of the skin graft models and the intensity of theimmune attack that the graft suffered.关键词
细胞毒性实验/移植排斥/免疫监测/流式细胞术/皮肤移植分类
医药卫生引用本文复制引用
梁伟潮,蒋泽生,汪燕,钟利民,潘明新..新西兰雌-雄兔皮肤移植模型体内细胞毒性实验[J].中国组织工程研究,2012,16(5):809-812,4.基金项目
国家自然科学基金资助项目(30972825)"供体抗原特异性体内免疫状态体内定量检测的基础研究". （30972825）
