中国组织工程研究2012，Vol.16Issue(5)：818-822,5.DOI:10.3969/j.issn.1673-8225.2012.05.014
新型免疫状态定量监测皮肤移植模型小鼠的特异性
Specificity of new quantitative monitoring of immune status in skin transplantation model rats
摘要
Abstract
BACKGROUND:How to monitor the reci pient's immune status, and predict the occurrence of rejection after organ transplantation,in order to adjust the dosage of immunosuppressive agents is the important issue faced currently.OBJECTIVE: To investigate antigen-specificity of new quantitative monitoring of immune status in skin transplantation model ratsMETHODS: The C57? BALB/c skin graft injection model was established, the models were injected with C57/BALB/c mixedsplenocytes suspension and third party DBA/BALB/c mixed splenocytes suspension respectively. The proportion of mixed cellsuspension and killing rate of donor cells were detected after cells injection. The homologous series skin transplantation controlgroup was setted, and compared with immunocompromised nude mice control group and immunosuppressant control group.RESULTS AND CONCLUSION: The C57/BALB/c suspension in model group and C57/BALB/c in immunosuppressant group hada strong specific cytotoxicity on C57 splenocytes of the donors. The specific cytotoxicity of C57/BALB/c suspension inimmunosuppressant group was weak, and there was no obvious specific cytotoxicity in the cells at 2-4 hours after third partyDBA/BALB/c mixed splenocytes injection. However, the cells of immunocompromised nude mice had no specific cytotoxicity. Theantigen-specific immune status detection method established in the experiment can detect the immune status of skin transplantrecipients rapidly.关键词
羧基荧光素乙酰乙酸琥珀酰亚胺酯/流式细胞仪/细胞毒性试验/移植排斥/细胞示踪/免疫监测分类
医药卫生引用本文复制引用
潘明新,钟利民,汪艳,高毅,蒋泽生..新型免疫状态定量监测皮肤移植模型小鼠的特异性[J].中国组织工程研究,2012,16(5):818-822,5.基金项目
国家自然科学基金资助项目(30972825),供体抗原特异性体内免疫状态体内定量检测的基础研究. （30972825）
