中国组织工程研究2012，Vol.16Issue(6)：951-957,7.DOI:10.3969/j.issn.1673-8225.2012.06.001
超顺磁性氧化铁纳米颗粒体外标记兔骨髓间充质干细胞的安全性以及MRI成像特征
Security and magnetic resonance imaging features of superparamagnetic iron oxide nanoparticles labelling rabbit bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: The traditional methods to label stem cells often need histopathology examination. It can not observe the whole experiment process dynamically and is not suitable for clinical research.OBJECTIVE: To observe the biological characteristics of rabbit bone marrow mesenchymal stem cells (BMSCs) labeled by superparamagnetic iron oxide (SPIO) nanoparticles in vitro, and to observe the magnetic resonance imaging (MRI) features of labeled BMSCs in vitro.METHODS: Rabbit BMSCs were cultured and expanded by using red blood cells lysis and adherent in vitro. Rabbit BMSCs were labeled with SPIO of different concentrations (100, 50, 25, 12.5 mg/L) combined with polylysine (PLL) (0.75 mg/L). The MRI imaging features of labeled rabbit BMSCs were observed by MRI scanning.RESULTS AND CONCLUSION: The rabbit BMSCs labeled with 25 mg/L SPIO combined with 0.75 mg/L PLL were more efficient and safe. This level of concentration could not influence on cells activity and could not influence the osteogenic and adipogenic differentiation capacity of labeled rabbit BMSCs obviously. MRI scanning on T2 * WI sequences can show the SPIO nanoparticles labeled rabbit BMSCs significantly and effectively.关键词
超顺磁性氧化铁纳米颗粒/骨髓间充质干细胞/标记/磁共振/安全性分类
医药卫生引用本文复制引用
孟增东,邱伟,胡彪,刘伟,戴敏芳,雷云坤..超顺磁性氧化铁纳米颗粒体外标记兔骨髓间充质干细胞的安全性以及MRI成像特征[J].中国组织工程研究,2012,16(6):951-957,7.基金项目
2010云南省应用基础研究基金项目(2010CD195),课题名称:SPIO标记兔BMSCs移植治疗早期股骨头缺血性坏死的MRI活体示踪研究. （2010CD195）
