中国组织工程研究2012，Vol.16Issue(6)：958-962,5.DOI:10.3969/j.issn.1673-8225.2012.06.002
物理联合化学或化学方法处理去抗原异种松质骨支架与骨髓间充质干细胞的细胞相容性
Cytocompatibility of bone marrow mesenchymal stem cells and antigen-extracted xenogeneic cancellous bone scaffold treated by physiochemical or chemical method
摘要
Abstract
BACKGROUND: Antigen-extracted xenogeneic cancellous bone has been shown to be characterized by well physicochemical performance and three-dimensional stereo porous structure, however, the biocompatibility and safely still need to be further studied in clinical application.OBJECTIVE: To study the cytocompatibility of bone marrow mesenchymal stem cells (BMSCs) and antigen-extracted xenogeneic cancellous bone scaffold treated by chemical or physicochemical method.METHODS: The gradient density centrifugation was used to separate and cultivate sheep BMSCs, the influence of antigen-extracted xenogeneic cancellous bone scaffold materials treated by chemical or physicochemical method on the proliferation of the BMSCs were measured by four thiazole salt colorimetric experiment (MTT method). The third generation of BMSCs were taken and seeded on two scaffolds, the morphology, adhesion, growth and proliferation of the cells on the two scaffold materials were observed by inverted phase contrast microscope and scanning electron microscope.RESULTS AND CONCLUSION: The cytotoxicity was 0 or 1 level in the physics joint chemical group, the cells exhibit well adhesion, proliferation and growth, cell activity was not affected by the material. The cytotoxicity was 3 level in chemical group, the cells were restrained, no cell adhesion in scaffold pores. The antigen-extracted xenogeneic cancellous bone materials treated with physical combined with chemical methods have good cytocompatibility to BMSCs. However, the scaffold materials treated by chemical methods have poor biocompatibility and it is not qualified for the safety standards of biological materials.关键词
异种骨/支架材料/骨髓间充质干细胞/细胞相容性/细胞毒性分类
医药卫生引用本文复制引用
许慧芬,何惠宇,唐小雪..物理联合化学或化学方法处理去抗原异种松质骨支架与骨髓间充质干细胞的细胞相容性[J].中国组织工程研究,2012,16(6):958-962,5.基金项目
国家自然科学基金项目(81060088),课题名称:三维打印构建组织工程化牙槽骨的实验研究 （81060088）
自治区自然科学基金项目(2011211A073),课题名称:荧光蛋白标记的组织工程骨修复颌骨缺损及其成骨能力的观测研究. （2011211A073）
