中国组织工程研究2012，Vol.16Issue(6)：963-967,5.DOI:10.3969/j.issn.1673-8225.2012.06.003
人骨髓间充质干细胞和汗腺细胞的体外分离与培养
Isolation and culture of human bone marrow mesenchymal stem cells and sweat gland cells in vitro
摘要
Abstract
BACKGROUND: In vitro research on the culture and identification of bone marrow mesenchymal stem cells (BMSCs) and sweat gland cells can provide the foundation for the feasibility of BMSCs regenerating sweat gland.OBJECTIVE: To study the effective method on isolation and culture of sweat gland cells and human BMSCs in vitro.METHODS: The BMSCs were isolated and cultured from adult bone marrow by adherent method, and then expanded and identified in vitro. Sweat gland cells were separated from human full thickness burnless skin using collagenase digestion method, and expanded and identified.RESULTS AND CONCLUSION: The separated and cultured BMSCs showed spindle-shaped and strong refraction under inverted microscope. Immunocytochemistry staining shows that CD29, CD44, CD105 were positive in BMSCs, surface markers CD34 and CD45 of hematopoietic stem cells were negative. Sweat gland cells showed flat polygonal, and surface markers cytokeratin (CK) 7, CK8, CK18, CK19 and carcinoembryonic antigen were positive in sweat gland cells. It is feasible to separate and culture BMSCs with adherent method and to isolate and culture sweat gland cells with collagenase digestion method.关键词
汗腺细胞/骨髓间充质干细胞/培养/分离/鉴定分类
医药卫生引用本文复制引用
艾丽,翁立新,孙同柱,孙虹..人骨髓间充质干细胞和汗腺细胞的体外分离与培养[J].中国组织工程研究,2012,16(6):963-967,5.基金项目
内蒙古自治区教育厅科学研究项目(NJZY07093),课题名称:诱导成体干细胞向汗腺细胞表型转化的实验研究. （NJZY07093）
