中国组织工程研究2012，Vol.16Issue(6)：1003-1006,4.DOI:10.3969/j.issn.1673-8225.2012.06.012
离子型谷氨酸受体拮抗剂MK-801浓度与全脑缺血再灌注大鼠海马内源性神经干细胞的增殖
Effect of ionotropic glutamate receptor antagonists MK-801 on the proliferation of endogenous neural stem cells in rats with global cerebral ischemia-reperfusion injury
摘要
Abstract
BACKGROUND: After cerebral ischemia-reperfusion, excessive excitatory amino acids can activate endogenous neural stem cells (NSCs) through N-methyl-D-aspartic acid (NMDA) receptor and can repair nerve cells by the proliferation and differentiation of endogenous NSCs but damage nerve cells by intracellular calcium overload at the same time.OBJECTIVE: To study the effect of NMDA receptor antagonist MK-801 on the proliferation of endogenous NSCs of global cerebral ischemia-reperfusion rat hippocampus.METHODS: The SD rats were divided into control group, operation group and different concentrations of MK-801 group (0.2, 0.4, 0.6, 0.8,1.0, 1.2 mg/kg). The rats were first treated with lateral ventricle intubation, and 3 days later, four-vessel occlusion was used to establish global cerebral ischemia-reperfusion model, except the control group. Different doses of MK-801 were intracerebrally ventricularly injected at 30 minutes before cerebral ischemia. The same concentration of normal saline was used in control group and operation group. Immunohistochemistry and RT-PCR technique were used to detect nestin positive cells and mRNA level expression.RESULTS AND CONCLUSION: When the concentration of MK-801 was under 0.8 mg/kg, the protein and mRNA expression of nestin was high and there was no difference between MK-801 group and operation group (P > 0.05). When the concentration of MK-801 was equal to 0.8 mg/kg, the expression of nestin gene and protein was decreased significantly with MK-801 increasing (P < 0.05). MK-801 can not only inhibit calcium overload but also has good effect on stimulating neural stem cell proliferation at the concentration of 0.6 mg/kg.关键词
离子型谷氨酸受体拮抗剂/脑缺血/再灌注/神经干细胞/MK-801分类
医药卫生引用本文复制引用
袁国艳,任铭新,郭义威,周立..离子型谷氨酸受体拮抗剂MK-801浓度与全脑缺血再灌注大鼠海马内源性神经干细胞的增殖[J].中国组织工程研究,2012,16(6):1003-1006,4.基金项目
2009年度新乡医学院第七批省级重点学科开放课题资助项目(ZD200923) （ZD200923）
2011年新乡医学院重点研究领域招标课题项目(ZD2011-14). （ZD2011-14）
