中国组织工程研究2012，Vol.16Issue(6)：1075-1079,5.DOI:10.3969/j.issn.1673-8225.2012.06.028
低强度脉冲超声波对SD大鼠前软骨干细胞增殖及细胞外基质mRNA表达的影响
Effect of low-intensity pulsed ultrasound on cell proliferation and the mRNA expression of extracellular matrix of Sprague-Dawley rat precartilagious stem cells
张迪 1祁军 1江渟
1李昆朋 1郭风劲1
作者信息
- 1. 华中科技大学同济医学院附属同济医院骨科,湖北省武汉市,430030
- 折叠
摘要
Abstract
BACKGROUND: Studies show that low-intensity pulsed ultrasound can improve the healing of bone fracture and regulate the cell proliferation and extracellular matrix secretion of cartilage.OBJECTIVE: To identify the effect of low-intensity pulsed ultrasound on cell proliferation and extracellular matrix secretion of precartilagious stem cells (PCSCs)METHODS: The precartilagious stem cells were isolated from the neonatal Sprague-Dawley (SD) rats and purified with magnetic activated cell sorting, identified by immunocytochemistry with the specific marker (FGFR-3). Passage 3 cells were divided into 4 groups: Blank control group, 5 minutes per day group, 12 minutes per day group and 20 minutes per day group. The cells were cultured for 24 hours continually after stimulated by LIPU. The cell proliferation was detected by CCK-8 method at the 1st, 3rd and 5th day. The total RNA was collected every 30 minutes after stimulated by low-intensity pulsed ultrasound, and the gene expression of collagen type II, Aggrecan, transforming growth factor |31 and Sox9 was detected by real-time polymerase chain reaction.RESULTS AND CONCLUSION: Low-intensity pulsed ultrasound could promote the proliferation of SD rats PCSCs, and compared with the blank control group on the first day only the 20 minutes per day group showed more obvious proliferation than the other groups, the difference was statistically significant (P < 0.05). Low-intensity pulsed ultrasound can also promote the gene expression of collagen type II, Aggrecan, TGF-β1 and Sox9 on SD rats PCSCs significantly. Higher amount of mRNA expression resulted from more stimulation, and the most effective group is 20 minutes per day group. Low-intensity pulsed ultrasound can promote the cell proliferation and extracellular matrix secretion of rat PCSCs. This effect may be mediated by TGF-β1 and Sox9 gene.关键词
低强度脉冲超声波/软骨组织工程/前软骨干细胞/细胞外基质/转化生长因子β1/Sox9分类
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张迪,祁军,江渟
,李昆朋,郭风劲..低强度脉冲超声波对SD大鼠前软骨干细胞增殖及细胞外基质mRNA表达的影响[J].中国组织工程研究,2012,16(6):1075-1079,5.
