中国组织工程研究2012，Vol.16Issue(7)：1157-1160,4.DOI:10.3969/j.issn.1673-8225.2012.07.005
碱性成纤维细胞生长因子可拮抗过氧化氢对软骨细胞的损伤
Basic fibroblast growth factors antagonize hydrogen peroxide damage to chondrocytes
赵文斌 1袁雪峰 1王茂朋 1张国良 1楚翔宇 1李文凯 1王江 1游洪波1
作者信息
- 1. 华中科技大学同济医学院附属同济医院骨科,湖北省武汉市,430030
- 折叠
摘要
Abstract
BACKGROUND: Whether basic fibroblast growth factor (bFGF) can antagonize hydrogen peroxide damage to chondrocytes isstill unclear.OBJECTIVE: To observe the antagonism effect of bFGF on the hydrogen peroxide-induced cell damage to rat knee-jointchondrocytes.METHODS: Chondrocyte apoptosis of Sprague Dawley (SD) rat knee-joint was induced by 0.05, 0.1, 0.2, 0.4, 0.8, 1.6 and3.2 mmol/L hydrogen peroxide. Effective concentration for induced apoptosis was chosen, and then 1 μg/L bFGF was added intoit for intervention.RESULTS AND CONCLUSION: The MTT data indicated that 0.2-1.6 mmol/L hydrogen peroxide could significantly induceapoptosis of chondrocytes of SD rat knee-joint. Apoptosis was increasing as the concentration was increased. The Hoechst33342staining and flow cytometry results showed that 1 μg/L bFGF could antagonise the hydrogen peroxide-induced apoptosis to ratknee-joint chondrocytes.关键词
过氧化氢/软骨细胞/碱性成纤维细胞生长因子/细胞凋亡分类
医药卫生引用本文复制引用
赵文斌,袁雪峰,王茂朋,张国良,楚翔宇,李文凯,王江,游洪波..碱性成纤维细胞生长因子可拮抗过氧化氢对软骨细胞的损伤[J].中国组织工程研究,2012,16(7):1157-1160,4.
