中国组织工程研究2012，Vol.16Issue(7)：1215-1219,5.DOI:10.3969/j.issn.1673-8225.2012.07.018
运动条件下健康人循环内皮祖细胞的数量和功能
Effect of exercise on number and activity of circulating endothelial progenitor cells in healthy subjects
摘要
Abstract
BACKGROUND: Whether exercise can influence the number and activity of endothelial progenitor cells (EPCs) is still unclear. OBJECTIVE: To observe the effect of exercise on number and activity of circulating EPCs in healthy subjects. METHODS: Twelve male healthy volunteers underwent acute dynamic treadmill exercise with (9.3±2.1) minutes. Flow cytometry analysis was used to evaluate the number of CD34 and KDR double-positive labeled circulating EPCs before and after exercise, and acetylated-low-density lipoprotein (LDL) and lectin fluorescent staining method were used to evaluate the number of cultured EPCs. The adhesion, migration and proliferation of EPCs were detected. In addition, the nitric oxide (NO), vascular endothelial growth factor (VEGF) and granulocyte-macrophage colony stimulating factor (GM-CSF) levels in plasma and secreted by circulating EPCs were measured before and after exercise.RESULTS AND CONCLUSION: Flow cytometry analysis showed that the number of circulating EPCs increased after exercise (P < 0.05). The acetylated-LDL and lectin fluorescent staining method indicated that the cultured EPCs also increased after exercise (P < 0.05). The proliferative and migratory activities of cultured EPCs were higher after exercise compared with before exercise (P < 0.05), but the adhesive activity did not change. Acute exercise also significantly enhanced plasma NO levels in healthy subjects (P < 0.05). There was a significant linear regression relationship between the enhanced plasma NO level and increased number or activity of circulating EPCs in healthy subjects (P < 0.05). Exercise can increase the number and activity of circulating EPCs in healthy subjects, which are related to the enhanced NO production in healthy subjects.关键词
内皮祖细胞/运动/内皮功能/心血管疾病/组织工程分类
医药卫生引用本文复制引用
杨震,靳亚非,陶军,赖光华,夏文豪,罗初凡,程飞,廖艳红,王洁梅,陈龙,廖新学..运动条件下健康人循环内皮祖细胞的数量和功能[J].中国组织工程研究,2012,16(7):1215-1219,5.基金项目
国家自然科学基金资助项目(u0732002,30800215,30770895),广东省自然科学基金博士启动项目(8451008901000793),广州市科技计划(2007Z3-E0241) （u0732002,30800215,30770895）
