中国组织工程研究2012，Vol.16Issue(7)：1241-1244,4.DOI:10.3969/j.issn.1673-8225.2012.07.024
急性心肌梗死模型大鼠经血管内皮生长因子121基因治疗后的血管新生
Angiogenesis in acute myocardial infarction rats after vascular endothelial growth factor 121 gene therapy
寿松涛 1林美光 2毛用敏 3曹丽 4张丽 2王佩显 2崔让庄3
作者信息
- 1. 天津医科大学总医院,急诊科,天津市,300052
- 2. 天津医科大学总医院,心脏科,天津市,300052
- 3. 天津市心血管病研究所,天津市,300051
- 4. 天津医科大学总医院,老年医学科超声室,天津市,300052
- 折叠
摘要
Abstract
BACKGROUND: Previous studies suggest that vascular endothelial growth factor 121 may be an optimal target gene for thetreatment of acute myocardial infarction.OBJECTIVE: To investigate effect of direct myocardial injection of adenovirus recombinant human vascular endothelial growthfactor 121 gene (Ad-hVEGF121) on myocardial infracted rat heart structure, function and angiogenesis.METHODS: Totally 78 male SD rats were randomly divided into the sham-surgery (n=18), acute myocardial infarction (n=24),Ad-VEGF121 (n=19) and normal saline (n=17) groups. Among them, left anterior descending coronary arteries of the latter threegroups were ligated to prepare acute myocardial infarction models and rats were randomly selected to receive Ad-hVEGF12 ornormal saline via three points in the cardiac muscle at the 10-15 minutes after ligation. The chest was exposed without ligation inthe sham-surgery group.RESULTS AND CONCLUSION: At 2 weeks after injection, cardiac ultrasound showed that, compared with the sham-surgerygroup, the number of new capillaries, body weight and left ventricular mass / body weight of the acute myocardial infarction,Ad-hVEGF121 and normal saline groups were obviously increased (P < 0.05 or P < 0.01), especially those received transfectedrAd-hVEGF12, had higher density of blood capillaries than those of the normal saline and acute myocardial infarction groups.However, there were no obviously differences between each group in infarct size, cardiac structure or functions. The directmyocardial injection of Ad-VEGF121 can significantly promote the formation of new blood vessels within the myocardium.关键词
血管内皮生长因子/基因治疗/急性心肌梗死/心功能/血管新生分类
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寿松涛,林美光,毛用敏,曹丽,张丽,王佩显,崔让庄..急性心肌梗死模型大鼠经血管内皮生长因子121基因治疗后的血管新生[J].中国组织工程研究,2012,16(7):1241-1244,4.
