中国组织工程研究2012，Vol.16Issue(7)：1245-1250,6.DOI:10.3969/j.issn.1673-8225.2012.07.025
深静脉血栓形成模型大鼠股静脉内皮组织中核转录因子kappa B1和组织因子的表达
Expression of nuclear factor kappa B1 and tissue factor in femoral venous endothelial tissue of the rat deep vein thrombosis model
摘要
Abstract
BACKGROUND: At present, the basic underlying molecular mechanism regulating the interactions among venous endothelial cells, platelets, leukocytes, and promoting local deep vein thrombosis microenvironment formation, still remains unclear, and there is no ideal method for early diagnosis of deep vein thrombosis. OBJECTIVE: To study the underlying role of nuclear factor kappa B1 and tissue factor in rats with deep vein thrombosis. METHODS: A total of 67 Sprague-Dawley rats were randomly divided into control group (n=10) and model group (n=57). Deep vein thrombosis model was established by a clamping and sewing method in femoral vein combined with cast fixation. The incidence and serious degree of thrombus were observed by dissecting rat femoral vein in different time points (2.5 and 25 hours after modeling). The model group was further divided into pre-thrombogenesis group (2.5 hours after modeling), thrombogenesis group (25 hours after modeling) and non-thrombogenesis group (25 hours after modeling). Then total RNA was extracted from the localized femoral venous endothelial tissue. The candidate genes, associated inflammation and thrombosis, were screened by a special gene chip. Then the gene expression of nuclear factor kappa B1 and tissue factor was further identified by real-time polymerase chain reaction. RESULTS AND CONCLUSION: Pre-thrombogenesis group had no thrombogenesis, while thrombogenesis group have 23 cases with thrombosis and non-thrombogenesis group have 22 cases without thrombosis. The results of gene chip hybridization analysis and real-time PCR found that the mRNA expression of nuclear factor kappa B1 and tissue factor in rat femoral vein endothelial tissue were significantly up-regulated at 2.5 hours after modeling (pre-thrombogenesis group was higher than control group) (P < 0.05), and continued up-regulating at 25 hours after modeling (thrombogenesis group was higher than the pre-thrombogenesis group, non-thrombogenesis group and control group) (P < 0.05). The results from present study indicate that up-regulating expressions of nuclear factor kappa B1 and tissue factor in local femoral venous endothelial tissue of rat deep vein thrombosis models may play a key role in initiating venous thrombosis.关键词
核转录因子kappa B1/组织因子/静脉内皮组织/深静脉血栓分类
医药卫生引用本文复制引用
李兴国,郑宏宇,李文,李宏昆,赵学凌,吴雪梅,王兵..深静脉血栓形成模型大鼠股静脉内皮组织中核转录因子kappa B1和组织因子的表达[J].中国组织工程研究,2012,16(7):1245-1250,6.基金项目
Supported by:the National Natural Science Foundation of China,No.30960389*,81060151*,81160236* （）
Joint Funds of Yunnan Sci-Tech Department and Kunming Medical College,No.2009cd159* （）
Yunnan New Key Product Development Projects,No.2010BC010* （）
Doctor Innovation Foundation of Kunming Medical College,No.2011D07*国家自然科学基金资助项目(30960389,81060151,81160236) （30960389,81060151,81160236）
云南省科技厅-昆明医学院联合项目(2009cd159) （2009cd159）
云南省重点新产品项目开发计划项目(2010BC010) （2010BC010）
昆明医学院博士研究生创新基金资助项目(2011D07). （2011D07）
