中国组织工程研究2012，Vol.16Issue(8)：1345-1348,4.DOI:10.3969/j.issn.1673-8225.2012.08.004
聚乳酸/羟基磷灰石膜与人羊膜基质细胞联合构建骨组织工程细胞/支架复合体
Combination application of human amniotic mesenchymal cells and poly (L-lactic acid)/hydroxyapatite membranes for construction of cells/scaffold complexes in bone tissue engineering
摘要
Abstract
BACKGROUND: Previous study has shown that electrospun poly (L-lactic acid)/hydroxyapatite (PLLA/HA) membranes aresuitable for cellular adhesion and proliferation.OBJECTIVE: To investigate the feasibility of the human amniotic mesenchymal cells (hAMCs) and PLLA/HA membranesconstructing the complex of seed cells/scaffold in bone tissue engineering.METHODS: Effect of leaching liquor from the PLLA and PLLA/HA membranes on the proliferation of hAMCs was detected by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay method. The third passage of hAMCs were cultured in theosteogenic induce media containing PLLA and PLLA/HA membranes, and then were detected by histological andimmunofluorescence cytochemical staining.RESULTS AND CONCLUSION: The leaching liquor of the PLLA and PLLA/HA had no cytotoxicity on the hAMCs. After seededonto two kinds of the membranes, hAMCs proliferated actively and calcified nodules were observed. Cells in calcified noduleswere shown positive expression of collagen type I and alkaline phosphatase, and the number and maturity of the calcifiednodules in the PLLA/HA group were better than those of the PLLA group. It is showed that the hAMCs and PLLA/HA canconstruct cells/scaffold complex and show a great potential of application in bone tissue engineering.关键词
聚乳酸/羟基磷灰石/人羊膜基质细胞/成骨诱导/骨组织工程分类
医药卫生引用本文复制引用
钟金晟,梅芳,齐伟宏,杨小平..聚乳酸/羟基磷灰石膜与人羊膜基质细胞联合构建骨组织工程细胞/支架复合体[J].中国组织工程研究,2012,16(8):1345-1348,4.基金项目
国家自然科学基金项目(30471907),课题名称:CNT/PLA/HA 电纺丝纳米纤维支架在骨组织工程中的基础研究. （30471907）
