中国组织工程研究2012，Vol.16Issue(8)：1349-1353,5.DOI:10.3969/j.issn.1673-8225.2012.08.005
长循环超顺磁性氧化铁脂质体纳米微粒的制备及理化性质
Initial preparation and physico-chemical properties of long-circulating superparamagnetic iron oxide liposome nanoparticles
摘要
Abstract
BACKGROUND: Common superparamagnetic iron oxide nanoparticles (SPIO) have limitations to apply to the reticuloendothelial system (RES) rather than non-RES.OBJECTIVE: To study the preparation and physico-chemical property of long-circulating SPIO liposome nanoparticles as novel MR contrast agent.METHODS: The long-circulating SPIO liposome nanoparticles were prepared by three steps: ①preparation of long-circulating blank liposomes by film dispersion method, using DSPG and DSPE-PEG2000 with a molar ratio of 20 : 1; ②synthesis of lauric acid-stabilized SPIO nanoparticles; ③the two products above were dialyzed for 3 days. Then, physico-chemical property of long-circulating SPIO liposome nanooparticles was measured.RESULTS AND CONCLUSION:①?Laurie acid-stabilized SPIO nanoparticles, long-circulating blank liposomes and long-circulating SPIO liposomes appeared as circular particles with uniform size approximately 12 nm, 20 nm, 60 nm, respectively in the electron microscope. ②XRD analysis of SPIO nanoparticles: lauric acid-stabilized SPIO nanoparticles and long-circulating SPIO liposomes nanoparticles were face-centered cubic spinel structure of Fe3O4, and the crystalline particles were in good form. ③With the Malvern-3000HS, the measurements of long-circulating SPO liposomes were: di = 78.7 nm, peak area: 100%, peak number 1, dv = 76.7 nm, peak area: 100%, peak number 1, dn = 73.5 nm, peak area: 100%, peak number 1, polydispersity coefficient: 0.212. ④Standard curve of iron: A=0.194 7C+0.000 8, R2=0.998 2. The Fe concentration of long-circulating SPIO liposome was 6.721 3 g/L. ⑤As the iron concentration increases, T2 values of long-circulating SPIO liposome gradually decreased. Long-circulating SPIO liposomes were prepared with suitable sizes and distribution, and could be made into contrast agent for subcutaneous or intravenous injection.关键词
超顺磁性氧化铁/对比剂/纳米微粒/脂质体/药效学分类
医药卫生引用本文复制引用
杨蕊梦,许乙凯..长循环超顺磁性氧化铁脂质体纳米微粒的制备及理化性质[J].中国组织工程研究,2012,16(8):1349-1353,5.基金项目
国家自然科学基金资助项目(30870708) （30870708）
广州市医药卫生科技资助项目(20121A011023). （20121A011023）
