中国组织工程研究2012，Vol.16Issue(8)：1354-1358,5.DOI:10.3969/j.issn.1673-8225.2012.08.006
纳米粒子介导Egr-1DNA酶转染抑制移植静脉内膜增生
Transfection of early growth response gene-1 DNA enzyme mediated by nanoparticles inhibits vein graft intimal hyperplasia
摘要
Abstract
BACKGROUND: The method of gene has been admitted to prevent stenosis and occlusion of grafted vascular. But it is a hot spotof how to increase gene transfection efficiency.OBJECTIVE: To study the effect of partial transfection of exogenous early growth response gene-1 DNA enzyme (Egr-1 DNAenzyme, EDRz) mediated by nanoparticles (NP) on the intimal hyperplasia (IH) in grafted vein.METHODS: The EDRz was constructed. Nanoparticle EDRz complex was prepared with polylactic/poly glycolic acid (PLGA) andpolyvinyl alcohol (PVA). Autogenously vein graft model was established, and the 210 rats were divided into three groups randomly:(1) EDRz group, EDRz mediated by NP was transfected into the veins before anastomosis. (2) Empty vector group, the vein wastransfected by empty vector mediated by NP. (3) Control group, no transfection.RESULTS AND CONCLUSION: In EDRz group, the expression of mRNA and protein of Egr-1 gene in intimal was significantdecreased than that in other two groups (P < 0.05) and at the 7th, 14th and 28th days, the thickness of intimal hyperplasia wasdecreased than that in other two groups (P < 0.01), the apoptotic percentage of vascular smooth muscle cells in EDRz group wassignificant increased than that in other two groups (P < 0.05). EDRz expression can prevent intimal hyperplasia and promoteapoptosis of vascular smooth muscle cells after autogenous vein grafting.关键词
纳米粒子/DNA 酶/移植静脉/转染/血管平滑肌细胞分类
医药卫生引用本文复制引用
刘程伟,张雪松,王石,王树卿,田浩,程明勋,胡新华,辛世杰,段志泉..纳米粒子介导Egr-1DNA酶转染抑制移植静脉内膜增生[J].中国组织工程研究,2012,16(8):1354-1358,5.基金项目
国家自然科学基金资助项目(30801123,30872527). （30801123,30872527）
