中国组织工程研究2012，Vol.16Issue(8)：1413-1416,4.DOI:10.3969/j.issn.1673-8225.2012.08.018
聚乳酸/壳聚糖纳米纤维/纳米羟基磷灰石支架与兔软骨细胞的相容性
Biocompatibility between polylactic acid/chitosan nano-fiber/nano-hydroxyapatite scaffolds and rabbit chondrocytes
梁鸿志 1查振刚 2姚平 1吴昊 2刘宁 3李劼若 1郇松玮 2苗贵强 1赵剑豪2
作者信息
- 1. 暨南大学附属第一医院骨科
- 2. 暨南大学骨科疾病研究所,广东省广州市,510632
- 3. 暨南大学医学院生理学教研室,广东省广州市,510632
- 折叠
摘要
Abstract
BACKGROUND: Traditional scaffold materials have the disadvantages of strong hydrophobic property, lack of bioactive molecules for specific binding of cell surface receptors on their surfaces, easy to lead to aseptic inflammation by their acidic degradation products, and so on. The ideal scaffolds for cartilage tissue engineering can be prepared based on bionic principle and real structure and composition of cartilage.OBJECTIVE: To prepare the polylactic acid/chitosan nano-fiber/nano-hydroxyapatite (PLLA/CSNF/nHA) scaffolds, evaluate their biocompatibility rabbit knee chondrocytes, and explore their application in articular cartilage tissue engineering. METHODS: The PLLA/CSNF/nHA scaffolds were prepared by two steps separation technology. Chondrocytes from New Zealand rabbits were seeded on the scaffolds for compound culture. The cell growth was observed under inverted phase contrast microscope. Cell-scaffold compounds were cultured in 24-well plates for 5 days, and then the compounds were implanted under the skin of nude mice for 8 weeks.RESULTS AND CONCLUSION: The prepared PLLA/CSNF/nHA scaffolds had an appropriate three-dimensional porous structure. The porosity was 90% and the pore size was 300-450 μm. Chondrocytes in cell-scaffold compounds could secrete proteoglycan and type II collagen like natural cartilage tested by immunohistochemical staining and toluidine blue staining. It is indicated that PLLA/CSNF/nHA has a good biocompatibility with rabbit chondrocytes and can be used as a type of bio-engineered stent.关键词
聚乳酸/组织工程/软骨细胞/壳聚糖/羟基磷灰石/支架分类
医药卫生引用本文复制引用
梁鸿志,查振刚,姚平,吴昊,刘宁,李劼若,郇松玮,苗贵强,赵剑豪..聚乳酸/壳聚糖纳米纤维/纳米羟基磷灰石支架与兔软骨细胞的相容性[J].中国组织工程研究,2012,16(8):1413-1416,4.
