中国组织工程研究2012，Vol.16Issue(9)：1597-1600,4.DOI:10.3969/j.issn.1673-8225.2012.09.018
针刺损伤兔椎间盘退变模型组织病理变化及软骨样细胞的迁移规律
Histopathological changes and rule of migration associated with chondrocytes in acupuncture-induced intervertebral disc degeneration model in rabbits
摘要
Abstract
BACKGROUND: The notochordal cells will be replaced by chondrocytes along with the aggravation of degenerativeintervertebral disc, but the origin and migration rule of the chondrocytes of the model established by puncturing the annulusfibrosus are still unclear.OBJECTIVE: To observe the histopathological process of degenerative intervertebral disc model established by puncturing theannulus fibrosus, and to explore the origin and migration rule of the chondrocytes.METHODS: Twenty-four New Zealand white rabbits were divided into operation group and sham operation group. In theoperation group, lumbar discs of L2-3, L3-4, L4-5 and L5-6 of each rabbit were punctured by 16 gauge needle. In the sham operationgroup, the anterior surfaces of the lumbar discs were exposed without puncture.RESULTS AND CONCLUSION: The chondrocytes of the nucleus pulposus originated and migrated from the cartilage endplate.At the junction of the nucleus pulposus and the cartilage endplate, the chondrocytes departed from the cartilage endplate to thenucleus pulposus vertically. At the junction of the inner annulus fibrosus and the cartilage endplate, the chondrocytes migratedalong the collagen fibers from the cartilage endplate to the shrinked margin of the nucleus pulposus. The non-calcified layerthinned gradually, and the non-calcified layer/calcified layer ratio decreased.关键词
椎间盘退变/软骨细胞/软骨终板/纤维环穿刺/新西兰大白兔分类
医药卫生引用本文复制引用
王诗军,李淳德,孙浩林,刘宪义,朱天岳,李文..针刺损伤兔椎间盘退变模型组织病理变化及软骨样细胞的迁移规律[J].中国组织工程研究,2012,16(9):1597-1600,4.基金项目
国家自然科学基金面上项目(81071504) （81071504）
北京自然科学基金项目(7123231). （7123231）
