中国组织工程研究2012，Vol.16Issue(10)：1725-1728,4.DOI:10.3969/j.issn.1673-8225.2012.10.004
低频振动刺激骨髓基质干细胞修复骨缺损核因子受体激活剂/核因子受体激活剂配体/骨保护素通路变化的体内实验
Effects of low frequency vibration on osteogenesis of bone marrow stromal cells and receptor activator of nuclear factor-kappa B/receptor activator of nuclear factor kappa-B ligand/osteoprotegerin pathway
摘要
Abstract
BACKGROUND: There have been few studies regarding low frequency vibration stimulating osteogenic differentiation of bone marrow stromal cells in vivo. OBJECTIVE: To investigate the effects of different frequencies of vibration on receptor activator of nuclear factor-kappa B(RANK)/ receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG) pathway and preliminarily investigate the underlying mechanism.METHODS: Eighty New Zealand rabbi ts of bone defect s were establi shed. Af ter transplantation of complex of New Zealand bone marrow stromal stem cells and decal cified bone matri x into bone defect region, rat model s were randomly di vided into a control group and four vibration groups according to different vibration frequencies (12.5, 25, 50, 100 Hz). The vibration group received different frequencies of vibration for 5 weeks from day 7. After vibration, OPG mRNA and RANKL mRNA expression was detected. RESULTS AND CONCLUSION: Compared with the control group, OPG and RANKL mRNA expression was significantly up regulated in each vibration group (P < 0.05), in particular in 25 and 50 Hz vibration groups (P < 0.01), but the expression was significantly down regulated in the 100 Hz vibration group (P < 0.05). This suggests that vibration at a certain frequency can stimulate bone marrow stromal cells to repair bone defects, which occurs possibly because vibration can promote the up regulation of OPG mRNA expression, with the ideal vibration frequency of 25 and 50 Hz.关键词
低频振动/骨髓基质干细胞/骨保护素/核因子受体激活剂配体/体内实验分类
医药卫生引用本文复制引用
杜广宇,赵文志,何盛为,米立东,张路,孙传秀,孙雪刚..低频振动刺激骨髓基质干细胞修复骨缺损核因子受体激活剂/核因子受体激活剂配体/骨保护素通路变化的体内实验 [J].中国组织工程研究,2012,16(10):1725-1728,4.基金项目
国家自然科学基金项目(30970708),课题名称:低频振动刺激BMSCs 修复骨缺损生物模型研究. （30970708）
