中国组织工程研究2012，Vol.16Issue(10)：1737-1741,5.DOI:10.3969/j.issn.1673-8225.2012.10.007
体外诱导人骨髓间充质干细胞向Ⅱ型肺泡上皮细胞分化
In vitro induction of human bone marrow mesenchymal stem cells to differentiate into type Ⅱalveolar epithelial cells
陈寅 1马南 2梅举 2肖海波 2陆善伟 2徐怀阳 1钟竑
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作者信息
- 1. 上海交通大学医学院附属新华医院,崇明,心胸外科,上海市202150
- 2. 上海交通大学医学院附属新华医院心胸外科,上海市200092
- 折叠
摘要
Abstract
BACKGROUND: Al veolar epithelial type Ⅱ (AT Ⅱ) has been demonstrated to directly participate in liver recovery and can also alleviate the severity of pulmonary fibrosis in a rat model of pulmonary fibrosis. Embryonic stem cells (ESCs) can differentiate into AT Ⅱ in vitro, but the application of ESCs is confined by many factors. OBJECTIVE: To investigate the method and transformation rate of bone marrow mesenchymalstem cells (BMSCs) differentiation into AT Ⅱ. METHODS: The BMSCs were isolated from human bone marrow cells. BMSCs were co-cultured with human embryonic lung mesenchymal cells (MRC-5) in serum-free small ai rway growth medium (SAGM) and modi fied SAGM. The morphology of BMSCs was observed, and the expression of surfactant protein C (SPC) mRNA was detected by RT-PCR. In addition, the expression of SPC was also detected by immunofluorescence.RESULTS AND CONCLUSION: After the BMSCs were co-cultured with MRC-5 for 10 days, some of BMSCs began to turn into epithelioid cells in morphous. After 15 days, the expression of SPC mRNA was detected, but it was notincreased along with the prolongation of coculture days. Compared with SAGM, modified SAGM could significantly increase the expression of SPC mRNA (P < 0.05). BMSCs could differentiate into AT Ⅱ in SAGM or modi fied SAGM when co-culture with MRC-5 invitro at low transformation rate. Positive rate of SPC was only (3.15±0.69)%.关键词
人骨髓间充质干细胞/共培养/分化/Ⅱ型肺泡上皮细胞/表面活性蛋白C分类
医药卫生引用本文复制引用
陈寅,马南,梅举,肖海波,陆善伟,徐怀阳,钟竑
..体外诱导人骨髓间充质干细胞向Ⅱ型肺泡上皮细胞分化 [J].中国组织工程研究,2012,16(10):1737-1741,5.
