中国组织工程研究2012，Vol.16Issue(10)：1769-1772,4.DOI:10.3969/j.issn.1673-8225.2012.10.014
人脐带间质干细胞培养上清液可下调肝星状细胞转化生长因子β的表达
Human umbilical cord stromal stem cells-conditioned medium down-regulated the expression of transforming growth factor beta in rat hepatic stellate cells
摘要
Abstract
BACKGROUND: Human umbilical cord stromal stem cells are expected to become a new seed cell in the treatment of live cirrhosis. However, few studies of in vitro experiment have been reported. OBJECTIVE: To explore the effect of human umbilical cord stromal stem cells-co ndi tioned medium (USSC-CM) on the biologi cal activity of fibrosis in rat hepatic stellate cells (HSCs), and the acting target.METHODS: HSCs were seeded in the six orifice plate in proportion (1.0×105 /mL), then cultured without serum for 24 hours; 2 mL USSC-CM were added to treat HSCs as experimental group; while HSCs were treated with equivalent fresh medium as control group. The expression of transforming growth factor β1 (TGF-β1), collagen-α1 and T IMP-2 mRNA in HSCs were detected by RT-PCR. The expression of TGF-β1 protein was determined by Western blot. RESULTS AND CONCLUTION: The expression of TGF-β1, collagen-α1 and TIMP-2 mRNA in HSCs were down-regulated in experimental group compared to control group, and the tendency enhanced with time (USSC-CM treated 72 h > USSC-CM treated 48 h > control group). And the expression of TGFβ1 protein in HSCs were down-regulated in experimental group compared to control group and showed time dependent manner (control group > USSC-CM treated 48 h group > USSC-CM treated 72 h group, P < 0.05). Human umbilical cord stromal stem cells can inhibit biological activity of HSCs. And it may carry its point by focusing on the acting target related to TGF-β together with downstream production.关键词
肝星状细胞/脐带间质干细胞/转化生长因子β/培养液上清/体外实验分类
医药卫生引用本文复制引用
李明亮,姚志成,钟跃思,林楠,许瑞云,邓美海..人脐带间质干细胞培养上清液可下调肝星状细胞转化生长因子β的表达 [J].中国组织工程研究,2012,16(10):1769-1772,4.基金项目
广东省自然基金项目(8151008901000112),广东省自然基金博士启动项目(10451008901004752),国家自然青年科学基金项目(81000177,81000674). （8151008901000112）
