中国组织工程研究2012，Vol.16Issue(10)：1795-1798,4.DOI:10.3969/j.issn.1673-8225.2012.10.019
尿酸对人胎盘间充质干细胞增殖的影响
Effect of uric acid on proliferation of human placenta-derived mesenchymal stem cells
崔晶 1杨乃龙 1王静怡2
作者信息
- 1. 青岛大学医学院附属医院黄岛院区内分泌科,山东省青岛市2266003
- 2. 青岛市玛丽医院产科,山东省青岛市266003
- 折叠
摘要
Abstract
BACKGROUND: Uric acid (UA) plays different roles on different cells. But, what is the effect of UA on the proliferation of human placenta-derived mesenchymal stem cells (hPMSCs), promoting or suppression? OBJECTIVES: To observe the effect of UA on proliferation of hPMSCs, and to explore the relationship between time-effect and dose-effect.METHODS: The hPMSCs cultured in vi tro were used and divided into 6 groups: the control group and the UA group which contained 0.1, 0.2, 0.4, 0.6, 0.8 mmol/L UA. RESULTS AND CONCLUSION: MTT assay showed that the number of hPMSCs in 0.1, 0.2, 0.4, 0.6, 0.8 mmol/L UA group was more than that in the control group at the same time, especially in 0.8 mmol/L UA group; the number of hPMSCs in same concentration of UA was increasing following the time (time﹤5 d), and reached to peak at the 5th day. UA could accelerate the proliferation of hPMSCs and showed a time- and dose-dependent manner, and the effect of 0.8 mmol /L UA on the proliferation capacity of hPMSCs was stronger than others at the 5th day.关键词
尿酸/浓度/人胎盘间充质干细胞/增殖/细胞数目分类
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崔晶,杨乃龙,王静怡..尿酸对人胎盘间充质干细胞增殖的影响 [J].中国组织工程研究,2012,16(10):1795-1798,4.
