中国组织工程研究2012，Vol.16Issue(10)：1799-1802,4.DOI:10.3969/j.issn.1673-8225.2012.10.020
体外诱导胰腺干细胞向胰岛样细胞团的分化
In vitro induced differentiation of pancreatic stem cells into islet-like cell clusters
摘要
Abstract
BACKGROUND: Because of lacking of islet sources, the islet cells transplantation for the treatment of diabetes can not meet the clinical demand, so the differentiation of pancreas stem cells into islets in vitro has become a focus of the research. OBJECTIVE: To in vitro induce the mice pancreas stem cells into the islet-like cell clusters and to perform the relate measurement; to investigate the techniques and methods to differentiate the pancreas stem cells into the islets as well as the detection method. METHODS: The mice pancreas stem cells were obtained in vi tro, and then the joint inducer was used to induce the pancreas stem cells to differentiate into islets. The islet- like cell clusters were preformed with morphological observation, dithizone dyeing, RT-PCR and Western blot detection. RESULTS AND CONCLUSION: Through the observation of cell morphology and cell growth characteristics and immunocytochemistry staining, we obtained mice pancreas stem cells in vitro. The joint inducer was used to induce the mice pancreas stem cells to differentiate into islet-like cells, the cells were spherical with a more slender pedicle connected with the bottom, and the cells was stained iron red by dithizone dyeing. The results of RT-PCR and Western blot determined the expression of insulin mRNA and insulin protein of islet-like cells respectively. It confirms that the mice pancreas stem cells can be induced in vitro to differentiate into beta containing islet- like cell clusters.关键词
胰腺干细胞/诱导/胰岛样细胞团/干细胞培养/小鼠分类
医药卫生引用本文复制引用
岑妍慧,谢小薰,陈维平,何国珍,黄波,郭文文,肖燕子..体外诱导胰腺干细胞向胰岛样细胞团的分化 [J].中国组织工程研究,2012,16(10):1799-1802,4.基金项目
广西壮族自治区自然科学基金青年基金(2010GXNSFB013070),广西壮族自治区教育厅研究生科研创新课题(2010105981001D34),广西壮族自治区教育厅研究生科研创新课题(2011105981001M191). （2010GXNSFB013070）
