中国组织工程研究2012，Vol.16Issue(10)：1808-1812,5.DOI:10.3969/j.issn.1673-8225.2012.10.022
Dlx2基因过表达与前成骨细胞系MC3T3-E1成骨分化过程中的细胞凋亡和周期调控
Effects of Dlx2 overexpression on cell cycle regulation and apoptosis of preosteoblast cells MC3T3-E1 during osteogenic differentiation process
摘要
Abstract
BACKGROUND: Dlx2 plays an important role in the regulation of cranial neural crest cells migration and craniofacial skeleton development. However, the effects of D1×2 on apoptosis and cell cycle in osteoblast differentiation are not reported. OBJECTIVE: To investigate the effects of D1×2 overexpression on cell cycle regulation and apoptosis in MC3T3-E1 preosteoblastcells differentiation. METHODS: Anti-retroviral pMSCV-puro-Dlx2 was transfected into MC3T3-E1 cell s whi ch were cultured in mineralization induced fluid. MC3T3-E1-Dlx2 cell lines stably overexpressing Dlx2 were constructed. Cell lines of Dlx2 over-expression were determined by RT-PCR and western blot. Apoptosis was detected by flow cytometry after Annexin V/PI and PI/Rnase staining and cell cycle changes were detected by flow cytometry after PI/Rnase staining. RESULTS AND CONCLUSION: MC3T3-E1-D1×2 cell lines was successfully constructed. Cell cycle was blocked in G1/G0 phase (P < 0.05) and apoptosis was up-regulated (P < 0.05) in Dlx2 overexpression cell model. Dlx2 overexpression reduces cell proliferation and promotes cell differentiation to exercise functions.关键词
成骨细胞分化/Dlx2/基因/稳定过表达/细胞凋亡/细胞周期分类
医药卫生引用本文复制引用
孙昊,王旭东,代杰文,卢境婷,沈国芳..Dlx2基因过表达与前成骨细胞系MC3T3-E1成骨分化过程中的细胞凋亡和周期调控[J].中国组织工程研究,2012,16(10):1808-1812,5.基金项目
上海市重点学科建设项目(S30206) （S30206）
上海市科学技术委员会(基础重点)项目(10JC1408700) （基础重点）
上海市自然基金(10ZR1418000) （10ZR1418000）
上海市卫生局资助项目(2009077) （2009077）
上海交通大学医工(理)交叉研究基金(YG2010MS55). （理）
