中国组织工程研究2012，Vol.16Issue(10)：1833-1836,4.DOI:10.3969/j.issn.1673-8225.2012.10.027
骨髓间充质干细胞移植影响慢性脑缺血大鼠认知功能及海马区Nogo-A 、NgR 的表达
Transplantation of bone marrow mesenchymal stem cells improves cognitive function of rats with chronic cerebral ischemia and decreases Nogo-A and NgR protein expression in the hippocampus
摘要
Abstract
BACKGROUND: Transplantation of bone marrow mesenchymal stem cells can decrease Nogo-A and NgR expression in rats with spinal cord injury and cerebral infarction. OBJECTIVE: To investigate the effects of transplantation of bone marrow mesenchymal stem cells on cognitive function of rats with chronic cerebral ischemia and Nogo-A and NgR protein expression in the hippocampus. METHODS: Thi rty Sprague-Dawley rats were randomly divided into sham-surgery, model and bone marrow mesenchymal stem cells groups. Rats in the latter two groups were established into model of chronic cerebral ischemia by permanent ligation of bilateral common carotid artery. In the bone marrow mesenchymal stem cells group, at 7 days after chronic cerebral ischemia induction, bone marrow mesenchymal stem cells (1×107) isolated by ficoll density gradient centrifugation were transfused into rats via the tailvein. RESULTS AND CONCLUSION: Compared with sham-surgery group, the average escape latency was significantly shortened (P < 0.01), the number that rats passed through the platform was significantly increased (P < 0.05), and Nogo-A and NgR expression in the hippocampal ti ssue was significantly decreased (P < 0.05) in the bone marrow mesenchymal stem cells group. These findings suggest that transplantation of bone marrow mesenchymal stem cells protects spatial learning and memory abilities of rats with chronic cerebral ischemia. This effect is possibly related to decreased Nogo-A and NgR protein expression.关键词
骨髓间充质干细胞/细胞移植/慢性脑缺血/Nogo-A/NgR/认知分类
医药卫生引用本文复制引用
王燕琳,闫禹,宋波,张惠莉,龚光明,陈思,方慧,许予明..骨髓间充质干细胞移植影响慢性脑缺血大鼠认知功能及海马区Nogo-A 、NgR 的表达 [J].中国组织工程研究,2012,16(10):1833-1836,4.基金项目
河南省重大公益科研项目(091100910800). （091100910800）
