中国组织工程研究2012，Vol.16Issue(10)：1841-1845,5.DOI:10.3969/j.issn.1673-8225.2012.10.029
腺病毒介导的肝细胞生长因子/血管内皮生长因子双基因转染骨髓间充质干细胞移植治疗心肌梗死
Treatment of myocardial infarction by transplantation of bone marrow mesenchymal stem cells transfected by adenovirus-mediated vascular endothelial growth factor and hepatocyte growth factor
摘要
Abstract
BACKGROUND: The bone marrow mesenchymal stem cells (BMSCs) can differentiate into myocardial cells and promote angiogenesis. However, the cell factor excreted by BMSCs in the early period of transplantation could not promote the differentiation and regeneration. OBJECTIVE: To investigate the effects of BMSCs transplantation transfected by adenovirous mediated vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) gene therapy on the tissue repair and angiogenesis in rabbits with myocardial infarction. METHODS: Rabbit BMSCs were isolated, cultured and purified in vitro, then labeled with BrdU and transfected by adenovirus mediated VEGF and HGF gene. 50 rabbits were used to establish the acute myocardial infarction models. Four weeks later, 50 rabbit models were randomly divided into five groups: group A was injected with BMSCs/Ad.VEGF+HGF; group B was injected with BMSCs/Ad.HGF; group C was injected with BMSCs/Ad.VEGF; group D was injected with BMSCs; group E was control group, it was injected with the same concentration of serum-free IMDM medium. The differentiation and angiogenesis of BMSCs were observed at 4 weeks after transplantation. Doppler echocardiography was performed to detect the changes on heart function. RESULTS AND CONCLUSION: Except for control group, the heart function was obviously improved after transplantation in group A, B, C and D (P < 0.05), and the improvement was the best in A group. Some BrdU stained positive BMSCs could differentiate into the endothelial cells and incorporate into the coronary capillaries in the infracted region. Compared to the control group, the other four groups could facilitate the angiogenesis (P < 0.05), especially in the group A. BMSCs implantation combining with VEGF and HGF gene therapy can obviously promote the myocardial regeneration angiogenesis, and can improve the heart function.关键词
基因转染/骨髓间充质干细胞/血管内皮生长因子/肝细胞生长因子/移植分类
医药卫生引用本文复制引用
陈国祥,华平,熊利华,陈矩,潘德强..腺病毒介导的肝细胞生长因子/血管内皮生长因子双基因转染骨髓间充质干细胞移植治疗心肌梗死 [J].中国组织工程研究,2012,16(10):1841-1845,5.基金项目
广东省科技计划项目(2007B050200011) （2007B050200011）
广东省自然科学基金资助项目(7001619) （7001619）
广东省中山市科技局科技计划项目(20091A103). （20091A103）
