中国组织工程研究2012，Vol.16Issue(12)：2095-2098,4.DOI:10.3969/j.issn.1673-8225.2012.12.002
胶原改良聚乳酸电纺丝支架用于组织工程化输尿管的体外构建
A modified electrospun polylactic acid/collagen scaffold for ureteral tissue engineering reconstruction in vitro
摘要
Abstract
BACKGROUND: Polylactic acid is a widely used scaffold material, but its hydrophobicity and lack of cell recognition signal limit its application. OBJECTIVE: To investigate the feasibility of constructing a tissue engineered ureter using a modified polylactic acid/typeⅠ collagen compound scaffold in vitro. METHODS: Electrospinning polylactic acid naofibrous scaffolds were dipped into a acetic acid solution of type- collagen to Ⅰmake the collagen absorbed onto the nanofiber surface. Then ureter epithelial cells were seeded separately onto the modified electrospun polylactic acid naofibrous scaffolds or control scaffolds. RESULTS AND CONCLUSION: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results confirmed that there was higher cellular activity in the collagen modified compound scaffolds, which was superior to cells untreated electrospinning polylactic acid scaffold at each time point. Scanning electron microscope showed that the most part of the surface of the modified scaffold was covered with ureter epithelial cells at 5 days after seeding. The modified polylactic acid/type collagen compound scaffold can obviously increase adhesion, growth and proliferation of the seeded cells, and has potential applications in ureteral tissue engineering reconstruction.关键词
输尿管/电纺丝/聚乳酸/胶原/黏附/增殖/组织工程分类
医药卫生引用本文复制引用
许永德,符伟军,史建国,谭海颂,张旭..胶原改良聚乳酸电纺丝支架用于组织工程化输尿管的体外构建[J].中国组织工程研究,2012,16(12):2095-2098,4.基金项目
国家自然科学基金(81070555),北京市自然科学基金(2092029)和军队临床高新技术重点项目(413DG63J). （81070555）
