中国组织工程研究2012，Vol.16Issue(12)：2133-2136,4.DOI:10.3969/j.issn.1673-8225.2012.12.010
铁碳纳米粒介导顺铂对喉癌细胞敏感性的研究
Effect of carbon coated iron nanoparticles carrying diamminedichloroplatinum on the chemotherapy sensitivity of laryngocarcinoma cells
摘要
Abstract
BACKGROUND: Nanometer technique is one of the effective ways to improve chemotherapy sensitivity of tumor cells by refining dosage form and target action of chemotherapy. OBJECTIVE: To investigate the depression effect of carbon coated iron nanoparticles carrying diamminedichloroplatinum (CCINs-DDP) on Hep-2 laryngocarcinoma cells and expression of caspase 3 and survivin mRNA. METHODS: Hep-2 laryngocarcinoma cells were intervened by iron-carbon nanoparticles and (or) normal saline dispersion of cisplatin. Cells interfered with normal saline were selected as controls. RESULTS AND CONCLUSION: Methylthiazolyldiphenyl-tetrazolium bromide assay showed that cisplatin could inhibit cell growth of Hep-2 cells, and combined with the iron-carbon nanoparticles, the inhibitory effect was stronger. Cells were not adherent, appeared with slow proliferation and apoptosis. Reverse transcription-PCR results showed that caspase 3 mRNA level of the Hep-2 cells was significantly increased at co-cultured 5 days after treated with iron-carbon nanoparticles combined with cisplatin and simple treated with the cisplatin, but the expression of survivin mRNA was significantly decreased, which was more obviously after treated with iron-carbon nanoparticles combined with cisplatin, but simple application of iron-carbon nanoparticles had no effect on Hep-2 cells. It is indicated that CCINs-DDP can improve the chemotherapy sensitivity of Hep-2 laryngocarcinoma cells on the DDP and also improve therapeutic efficacy of chemotherapeutics.关键词
铁碳纳米粒/顺铂/喉癌/Hep-2细胞/化疗/敏感性/Caspase3/Survivin分类
医药卫生引用本文复制引用
朱力,侯楠,胡国华..铁碳纳米粒介导顺铂对喉癌细胞敏感性的研究[J].中国组织工程研究,2012,16(12):2133-2136,4.基金项目
四川省卫生厅立项项目(100107),课题名称:铁碳纳米粒介导顺铂对喉癌细胞的敏感性影响研究. （100107）
