中国组织工程研究2012，Vol.16Issue(12)：2175-2179,5.DOI:10.3969/j.issn.1673-8225.2012.12.020
聚酰胺-胺型树枝状高聚合物介导Survivin反义寡核苷酸抑制人肝癌裸鼠移植瘤生长的作用
Antitumour effects of survivin antisense oligonucleotide carried by polyamidoamine dendrimer liposome on hepatic cancer transplanted subcutaneously in nude mice
摘要
Abstract
BACKGROUND: Antisense oligonucleotide (ASODN) can inhibit survivin expression which induce tumor cell apoptosis. There are still some problems in malignant tumors gene therapy by survivin such as insufficient gene transfer, no long-term stable expression, enzyme degradation. OBJECTIVE: To evaluate the antitumour effects of survivin ASODN carried by polyamidoamine (PAMAM) dendrimer liposome on hepatic cancer transplanted subcutaneously in nude mice. METHODS: An in vivo model of hepatic cancer was established by injecting SMMC-7721 cells subcutaneously into the flanks of nude mice. The PAMAM dendrimer liposome and PAMAM dendrimer were mixtured with survivin-ASODN respectively to generate the transfection complex. The zeta potential and encapsulating effciency in vitro were determined. When the tumours were palpable, the mixture complex was directly injected into xenografts to observe the size of tumours. The expression of survivin in transplant tumour was measured. RESULTS AND CONCLUSION: The zeta potential of the PAMAM liposome-survivin-ASODN complex was higher than that of the PAMAM-survivin-ASODN complex (P < 0.05). There was no significant difference of envelopment ratio between PAMAM liposome-survivin-ASODN group and PAMAM-survivin-ASODN group (P > 0.05). The expression of survivin protein and weight in transplanted tumours for the PAMAM liposome-survivin-ASODN group were lower than those for PAMAM-survivin-ASODN group (P < 0.05). The PAMAM dendrimer liposome can delivery surviving ASODN into hepatic transplanted tumour cells effectively, reduce the expression of surviving and induce tumour cells apoptosis.关键词
聚酰胺-胺型树枝状高聚合物/脂质体/肝癌/反义寡核苷酸/Survivin分类
医药卫生引用本文复制引用
厉周,方素珍,韩帅,崔大祥,蔡寨,李强,朱卉娟,黄宗海..聚酰胺-胺型树枝状高聚合物介导Survivin反义寡核苷酸抑制人肝癌裸鼠移植瘤生长的作用[J].中国组织工程研究,2012,16(12):2175-2179,5.基金项目
广东省自然科学基金资助项目(9151051501000071),项目名称:阳离子树形分子脂质体介导Survivin反义寡核苷酸治疗肝癌的研究. （9151051501000071）
