现代科学仪器Issue(6)：130-132,3.
气相色谱-质谱法快速测定猪肉中的胆固醇
A Fast Determination of Cholesterol in Pork by Gas Chromatography-Mass Spectrometry
喻文娟1
作者信息
- 1. 上海交通大学分析测试中心，上海200240;上海交通大学农业与生物学院，上海200240
- 折叠
摘要
Abstract
A simple method for the determination of cholesterol in pork by external standard-gas chromatography-mass spectrometry has been established.The Single-factor experiment was employed to optimize some sample preparation procedures in the relevant Chinese Standard,determining that ethyl ether was the best extract among three solvants and the optimum saponification temperature and time were 70 °C and 50min respectively.Meanwhile,the methodological study showed that for cholesterol,R2 of linear regression was above 0.999;limit of detection（LOD） was 1.05 μg/g;the recoveries at different levels were all above 75%,and relative standard deviations（RSDs） were below 7%.The method possesses the characteristics of rapidity,simplicity and environmentally friendliness,the linearity,sensitivity,accuracy and precision,it can all met the requirements of daily analysis of cholesterol in pork.关键词
气相色谱法/质谱法/猪肉/胆固醇Key words
Gas chromatography/mass spectrometry（GC/MS）/Pork/Cholesterol分类
化学化工引用本文复制引用
喻文娟..气相色谱-质谱法快速测定猪肉中的胆固醇[J].现代科学仪器,2011,(6):130-132,3.
