新疆农业科学2011，Vol.48Issue(11)：2041-2045,5.DOI:65-1097/S.20111130.1822.013
白粉病胁迫对甜瓜同工酶谱的影响
Effects of Powdery Mildew Stress on Isoenzymes of Melon
摘要
Abstract
[Objective] To determine the relationship between isozymes of SOD, POD, CAT of melon seedlings and powdery mildew. [ Method ] The changes of three kinds of isozymes in susceptible variety of Jiashi melons and MR -1 of resistance variety before and after inoculating powdery mildew were analyzed by using PAGE electrophoresis. [Result] Activity of the 3 kinds of isoenzyes changed and POD emerged a new enzyme after inoculating powdery mildew three days compared with control: jiashi P4(Rf =0. 80) , MR -1 :P, (Rf = 0. 68 ) , and the changes of the activity of POD isozyme in Jiashi was violent than that in MR -1 during the treatment. The isoenzyes of CAT didn't produce new lane, but two peaks emerged after inoculating powdery mildew 3 days and 12 days. The activity change of SOD was weak compared with others, but the lane of S, (Rf = 0.68) had a trend that was higher than that of contrast in day 6 and were lower in day 9. [Conclusion] The defense enzymes SOD, CAT, POD can be used as a measure of indicators of resistance to powdery mildew in melon.关键词
白粉病/POD/SOD/CAT/同工酶Key words
powdery mildew/POD/CAT/SOD/isoenzyme分类
农业科技引用本文复制引用
樊月,谷俊霞,钟俐..白粉病胁迫对甜瓜同工酶谱的影响[J].新疆农业科学,2011,48(11):2041-2045,5.基金项目
新疆维吾尔白治区自然科学基金(2011211A013) （2011211A013）
新疆大学(XJU-SRT) （XJU-SRT）
