新疆农业科学2011，Vol.48Issue(11)：2067-2070,4.DOI:65-1097/S.20111130.2139.018
梨树苹果茎痘病毒PRINS检测中引物的设计及退火温度的测定
The Design of the Primer and the Testing of Annealing Temperature of ASPV PRINS Detection
摘要
Abstract
[ Objective ] In order to complete testing PRINS primer and annealing temperature measurement, five primers were used to amplify the purpose fragments separately. [Method] Korla Pear leaves identified with ASPV were selected as test materials, healthy pear leaves detected by RT - PCR were selected as negative control. Primers were designed by using the conserved regions of the coat protein of ASPV virus' complete genome sequence, which was part of the BLAST tool provided by NCBI. In addition, the distinctive characteristics of PRINS and the phenomenon that ASPV fragments overlapped with each other were also taken into consideration in the process of primer design. [ Result ] There was little influence between five primers. And clear bands were all showed on the purpose locations. [ Conclusion ] Therefore these five primers could be used directly for the next step of PRINS detection. It laid a good foundation for PRINS to detect pear virus.关键词
引物原位标记/引物/病毒/检测Key words
primed in situ labeling/primer/virus/detection分类
农业科技引用本文复制引用
吴庆丰,牛建新,李文慧,刘娜..梨树苹果茎痘病毒PRINS检测中引物的设计及退火温度的测定[J].新疆农业科学,2011,48(11):2067-2070,4.基金项目
国家自然科学基金项目(30360066) （30360066）
国家科技攻关计划引导项目(2003 BA546C) （2003 BA546C）
石河子大学自然科学与技术创新(500002125707) （500002125707）
