新疆农业科学2011，Vol.48Issue(11)：2071-2077,7.DOI:65-1097/S.20111130.2140.019
响应面法优化黄色短杆菌c-11产L-丝氨酸发酵培养基
Optimization of Fermentation Medium for L - serine Production by Brevibacterium flavum by Using Response Surface Methodology
摘要
Abstract
[ Objective ] The purpose of this paper was mainly to study the optimizing medium of Brevibacterium flavum in order to improve the production of L - serine. [ Method] Firstly, the influences of eight related factors were evaluated in fermentation medium by Plackett - Burman design to filter out the main factors. The eight related factors were sucrose, yeast extract, (NH4 )2SO4, KH2PO4, MgSO4, biotin, VB1 and CaCO3. The results showed that sucrose, yeast extract and (NH4)2SO4 were the main affecting factors. Secondly, the experimental design of steepest ascent was used to approach the best fermentation condition of main factors - sucrose, yeast extract, (NH4 )2SO4. Based on this result, a three - level three - factor central composite design was employed to determine the maximum L - serine production at optimum concentration of sucrose, yeast extract and (NH4)2SO4. The L - serine production was found to correlate to the three parameters that could be represented by second - order polynomial models. [ Result] The optimal values of the three parameters were determined as 83 g/L sucroes, 31 g/L yeast extract and 29 g/L ( NH4) 2 SO4. The L -serine production was 22.27 g/L. In theory, the actual experimental result was 22.65 g/L. They were in well agreement. [Conclusion] The production of L - serine was increased by 28. 11% in optimized fermentation medium.关键词
L-丝氨酸/黄色短杆菌/响应面法(RSM)Key words
L - serine/Brevibacterium flavum/response surface methodology分类
农业科技引用本文复制引用
杨慧,包慧芳,王宁,王炜..响应面法优化黄色短杆菌c-11产L-丝氨酸发酵培养基[J].新疆农业科学,2011,48(11):2071-2077,7.基金项目
新疆维吾尔自治区高技术研究发展计划(200910102) （200910102）
新疆维吾尔自治区重大专项(200740147-2) （200740147-2）
新疆维吾尔自治区科技支疆项目(201091227) （201091227）
新疆维吾尔自治区科技基础条件平台建设项目(PT0807) （PT0807）
