新疆农业科学2011，Vol.48Issue(12)：2217-2222,6.DOI:65-1097/S.20120109.2044.010
我国马铃薯甲虫生物防治技术研究进展
Progress in Biological Control Techniques of Colorado Potato Beetle in China
摘要
Abstract
[Objective]and Method ] By reviewing and summarizing the study progress in biological control of Colorado potato beetle in China in recent years, the adequate evidence and reference were provided about that area. [ Result ] Various chemicals originated from biological resource have been selected, whose effect against CPB could reach as high as 90. 0% at the time of 20 d after being used. In accordance withsurvey which was made in the northern part of Xinjiang, China, there were 46 sorts of predator of CPB and the main entomogenous fungi of CPB was Beauveria bassina which was distributed widely in occurrence area of CPB in China. The enterprise standard has been registered in which 30 billion spores per gram WP and 10 billion spores per gram OF against CPB have been developed. The result of field trial showed that effect could reach about 80 %. Several plant volatiles and aggregation pheromone were discovered, in which the addition of aggregation pheromone to the three - component blend increased the attraction percentage of both male (88. 33% ) and female beetles (83. 33% ). The breakthrough of application of RNA interference techniques has been achieved. The RNAi which is related with juvenile hormone biosynthesis and metabolism gene has been discovered for the first time all over the world. A great amount of dsRNA derived from 3 - hydroxy - 3 -methylglutaryl CoA reductase, mevalonate kinase, adenosylhomocysteinase, juvenile hormone esterase in L. decemlineata was obtained. Bacteria liquids contained adenosylhomocysteinase dsRNA showed lethal effect to the larvae when orally administrated to 2nd - instar larvae. The conclusion of the study made it possibility to develop the genes insecticide with RNAi. The cry3A gene and both cry3A and vhb genes the transgenic potato plants were constructed. These results of field trial and laboratory bioassay indicated that was a high insect -resistant transgenic line with elite agronomic traits, and could be used as germplasm to produce the novel insect - resistant cultivars. [ Conclusion ] Because of their characteristics such as no pollution, the practicality and the advanced character etc. , the new type biological control techniques could substitute for the chemical methods, which could not only well control the CPB but also have much more significance in resistance management.关键词
马铃薯甲虫/生物防治技术/研究进展/中国Key words
Colorado Potato Beetle/biological control technique/study progress/ China分类
农业科技引用本文复制引用
郭文超,邓春生,李国清,邓建宇,姜卫华,吴家和,王佩玲,谭万忠,张青文..我国马铃薯甲虫生物防治技术研究进展[J].新疆农业科学,2011,48(12):2217-2222,6.基金项目
国家公益性行业(农业)科研专项"马铃薯甲虫持续防控技术研究与示范"(200803024) （农业）
"入侵昆虫综合防控技术研究与示范推广"(201103026) （201103026）
农业部西北荒漠绿州农作物有害生物综合治理重点实验室项目 （）
