新疆农业科学2011，Vol.48Issue(12)：2266-2272,7.DOI:65-1097/S.20120109.2055.019
二次回归正交旋转组合设计优化新疆白皮蒜鳞茎盘组织培养的研究
Optimization of Tissue Culture in Xinjiang White Garlic by Using Quadratic Regression Orthogonal Rotation Combination Design
摘要
Abstract
[Objective] The purpose of this project aimed to study the effects of the plant hormones on Xinjiang white garlic bulb disadventitious shoots growth in order to provide a theoretical basis for rapid propagation system. [ Method ] The experiment adopted quadratic regression orthogonal rotation combination design, design of different IB A, NAA,6 - BA level and their combination. [Result] The result showed that the primary and secondary sequence of the factors that affect adventitious shoots growth is: NAA > 6 - BA > IBA, among which, the interaction between NAA and IBA was significant but with negative effects, so in the highest concentration prediction, IBA is removed to reduce interaction to Xinjiang white garlic bulb disc adventitious shoots growth effects. The single 6 - BA has more obvious effects (usually positive effect) than other factors. [ Conclusion ] The best ratio of hormone for Xinjiang white garlic bulb disc adventitious shoots growth was; NAA concentration was 2 mg/L, 6 - BA concentration was 7. 5 mg/L. In this condition Xinjiang white garlic disc adventitious shoots growth can reach the maximum, which was 39.22.关键词
大蒜/鳞茎盘/不定芽/二次回归正交旋转组合设计Key words
Allium sativum L. / bulb disc/ adventitious shoot/ quadratic regression orthogonal rotation combination design分类
农业科技引用本文复制引用
董瑞,唐明磊,林辰壹,熊祖华,高杰..二次回归正交旋转组合设计优化新疆白皮蒜鳞茎盘组织培养的研究[J].新疆农业科学,2011,48(12):2266-2272,7.基金项目
国家公益性行业(农业)科研专项(200903018-7) （农业）
