畜牧兽医学报2011，Vol.42Issue(7)：974-980,7.
乳房炎奶牛金黄色葡萄球菌毒素基因的检测及PFGE分型研究
Virulence Genes and PFGE Profiles of Staphylococcus aureus Isolated from Cows with Subclinical and Clinical Mastitis
摘要
Abstract
The aim of the present study was to investigate toxin genes properties and PFGE profiles of S. aureus strains (SA) isolated from cows with subclinical mastitis and clinical mastitis.For this purpose, milk samples were sterilely collected from Chinese Holstein cows with subclini-cal and clinical mastitis. SA strains or methicillin-resistant S. aureus (MRSA) strains were confirmed by multiplex PCR based on nuc and mecA genes. SA isolates were recovered and characterized by kinds of toxin genes of SA (Ses, Ets, TSST-1 and PVL genes) with PCR and PFGE assays. Results were as follows: 69 S. aureus isolates were recovered from 19. 3% subclinical mastitis samples (43 isolates) and 14.8% clinical mastitis samples (26 isolates), in which 5 isolates from clinical mastitis were wecA-positive S. aureus. From clinical mastitis isolates, SEA, SEB, SED, SEJ and PVL genes were detected with the rate of 3. 8% (1 isolate) , 11. 5% (3 isolates) , 19. 2% (5 isolates) , 7. 7% (2 isolates) and 31. 2% (10 isolates), respectively, whereas SEA and PVL were detected from subclinical mastitis isolates with the rates of 7. 0% (3 isolates) and 84. 1% (37 isolates) , respectively. The results implied that Ses may be important pathogenic genes of S. aureus strains in the cows with clinical mastitis, while PVL may be key pathogenic gene for the cows with subclinical mastitis. Fifty pulsotypes and seven PFGE lineage groups from A to G were obtained by PFGE, and S. aureus strains isolated from the same source were classified into the same cluster. MRSA strains were only isolated from cows with clinical mastitis. The detection rates of PVL genes in cows with subclinical mastitis were 2. 7 times higher than that with clinical mastitis. The distribution of virulence genes in S. aureus isolates was distinctly different between the cows with subclinical mastitis and clinical mastitis via PFGE assays.关键词
奶牛/金黄色葡萄球菌/mecA基因/毒素基因/PFGE分型Key words
cows/ S. aureus/ mecA/ virulence genes/ PFGE分类
医药卫生引用本文复制引用
王新,俞英,韦艺媛,张静,周婷,梁珍娟,杨保伟,席美丽,夏效东,孟江洪..乳房炎奶牛金黄色葡萄球菌毒素基因的检测及PFGE分型研究[J].畜牧兽医学报,2011,42(7):974-980,7.基金项目
长江学者讲座教授奖励计划项目(Z111020001) （Z111020001）
博士科研启动费(01140407) （01140407）
教育部基础科研业务项目(2011JS006) （2011JS006）
转基因生物新品种培育科技重大专项(2009ZX08009-146B) （2009ZX08009-146B）
北京市农业局试验示范项目(20100222) （20100222）
"948"项目(2010-C14) （2010-C14）
