畜牧兽医学报2011，Vol.42Issue(7)：1007-1014,8.
磺胺二甲嘧啶快速直接竞争ELISA试剂盒的研制及应用
Development and Application of Rapid Direct Competitive ELISA Kit for Sulfamethazine
摘要
Abstract
The aim of this study was to develop the direct ELISA kits based on polyclonal antibody to detect the sulfamethazine in pork liver, pork and chicken, and the results were confirmed by HPLC. Sulfamethazine was spiked to pork liver, pork and chicken and was detected by the ELISA and HPLC. The sensitivity, specificity, precision, veracity and validity of the kits were verified. Its detection limit was 0. 47 ng · mL-1 with a concentration range from 0. 5 to 50 ng · mL-1. The mean intra-assay variability and inter-assay was 7. 4% and 9. 27%, respectively. The detection limits were 3. 91, 4. 81 and 1. 59 μg · kg-1 in pork liver, pork and chicken. The recovery was between 85%-110% when spiked to samples at 10. 0-50. 0 μg · kg-1 , which is adaptable to the veterinary use. The kit's detection time is 12. 6 min, and can be stored at 4 °C at least 6 months. The kit is satisfying rapid screen of SMZ residue in pork liver, pork and chicken.关键词
磺胺二甲嘧啶/ELISA试剂盒/猪肝/猪肉/鸡肉Key words
sulfamethazine/ ELISA kit/ pork liver/ pork/ chicken分类
农业科技引用本文复制引用
龚云飞,王唯芬,张明洲,陈宗伦,李沐洁,奚茜..磺胺二甲嘧啶快速直接竞争ELISA试剂盒的研制及应用[J].畜牧兽医学报,2011,42(7):1007-1014,8.基金项目
浙江省重大科技专项(2006C12102) （2006C12102）
杭州市重大科技创新专项(20083212A25) （20083212A25）
