畜牧兽医学报2011，Vol.42Issue(7)：1027-1031,5.
部分中国荷斯坦种公牛脊柱畸形综合征携带状况的检测和分析
Use of a Mismatch Amplification Mutation Assay PCR Method to Detect the Complex Vertebral Malformation in Some Chinese Holstein Sires
摘要
Abstract
This experiment was conducted to test some Chinese Holstein sires for complex vertebral malformation (CVM). In this study, a simple, rapid PCR mismatch amplification mutation assay (PCR- MAMA) was developed to detect the mutation allele. Out of 154 tested Holstein sires, 24 sires (15. 58%) were identified to be CVM carriers by PCR- MAMA. The results indicate that all the Chinese Holstein sires should be tested for CVM.关键词
脊柱畸形综合征/错配PCR突变分析技术/遗传疾病Key words
complex vertebral malformation (CVM)/ PCR-MAMA/ inherited defect分类
农业科技引用本文复制引用
王帅,赵学明,朱化彬,杜卫华,王栋,郝海生,王宗礼..部分中国荷斯坦种公牛脊柱畸形综合征携带状况的检测和分析[J].畜牧兽医学报,2011,42(7):1027-1031,5.基金项目
现代农业产业技术体系建设专项资金(nycytx-10,CARS-37) （nycytx-10,CARS-37）
引智项目(Y20100326004) （Y20100326004）
转基因专项(2009ZX08010-015B （）
2009ZX08011-031B) （）
