畜牧兽医学报2011，Vol.42Issue(10)：1351-1356,6.
FSH调节仔猪睾丸支持细胞GDNF表达的信号机制
The Signaling Mechanism of GDNF Expression in Sertoli Cells of Piglet Testis Regulated by FSH
摘要
Abstract
This experiment was conducted to study the mechanism of FSH regulating GDNF expression in Sertoli cells. Different factors (inhibitor or activator) of signal pathway was added to Sertoli cells in vitro cutured, RT-PCR and Western blot were used to detect the effect of FSH on GDNF expression. The result showed that; (1) FSH(50 ng · mL-1) could active ERK1/2 prominently along with a maximum stimulation at 2 h, the ERK1/2 reached a peak activity at 30 min. (2)dbcAMP(100 μmol · L-1) could not only increase the activity of ERK1/2 but also enhance the level of GDNF protein and mRNA with a maximum stimulation at 2 h. (3) While adding U0126 (10 μmol · L-1, MEK1/2 inhibitor) or H89 (10 μmol · L-1, PKA inhibitor) to culture medium, the results showed that the expression level of GDNF protein, mRNA and activity decreased apparently as compared with that in the cells treated with FSH alone. (4) The FSH-activated ERK1/2 in the cultured SC was time-dependent, with a peak activity at 20 min. The activation of ERK1/2 by FSH was blocked by PKA inhibitor H89. These results indicate that FSH can activate ERK1/2 via cAMP-PKA, which increase the expression of GDNF in Sertoli cell.关键词
FSH/支持细胞/GDNF蛋白/信号传导Key words
FSH/Sertoli cell/GDNF/signal transduction分类
农业科技引用本文复制引用
孙燕,王鲜忠,吴建云,朱峰伟,向阳,杨炜蓉,张家骅..FSH调节仔猪睾丸支持细胞GDNF表达的信号机制[J].畜牧兽医学报,2011,42(10):1351-1356,6.基金项目
中央高校基本科研业务费专项资金(XDJK2009B036) （XDJK2009B036）
西南大学博士启动基金(08BSr03) （08BSr03）
