畜牧兽医学报2011，Vol.42Issue(10)：1477-1484,8.
利用抑制消减杂交技术筛选鹅就巢行为相关基因
Identification of Broodiness-Related Geese Genes by Suppression Subtractive Hybridization
摘要
Abstract
The aim of this study was to construct broodiness and laying geese cDNA libraries with SSH, and to identify differentially expressed genes between laying and broody phases. Ovary in broody and laying phases was ascribed to tester and driver for reciprocal SSH procedures. cDNA libraries of specifically or highly expressed genes in broodiness and laying were obtained after two-step hybridization. Positive clones from libraries were randomly selected to validate with PCR. Sequently, 654 positive clones were selected to sequence, qualify, cluster, assemble and compare in GenBank using BLASTn. 166 differentially expressed gene fragments from two libraries showed homology to other known sequences, including 92 functional genes. The expression profiles showed some broodiness-specified genes invovled in apoptosis, cell component, hormones-related signal transduction, and some genes involved in metabolism and cell defense in the laying-specified genes library; some ESTs belonged to unknown or hypothetical protein. The result indicate that three genes(prolactin receptor, estrogen receptor α and anti-mullerian hormone receptor Ⅱ) in broodiness-specified library are associated to trigger or maintain broodiness in geese based on biological function and signal transduction. The result provide the basis for future revealing the mechanism on broody behavior.关键词
鹅/就巢行为/卵巢/抑制消减杂交技术/表达序列标签Key words
geese/broodiness/ovary/SSH/ESTs分类
农业科技引用本文复制引用
郭军,汤青萍,章双杰,马月辉,陆火林,苏建东,邹剑敏,陈宽维,李慧芳..利用抑制消减杂交技术筛选鹅就巢行为相关基因[J].畜牧兽医学报,2011,42(10):1477-1484,8.基金项目
国家科技支撑计划项目(2008BADB2B08) （2008BADB2B08）
江苏支撑计划(BE2010370) （BE2010370）
现代农业产业技术体系建设专项资金(nycytx-42-G1) （nycytx-42-G1）
