畜牧兽医学报2011，Vol.42Issue(11)：1543-1549,7.
利用微卫星DNA标记分析槟榔江水牛群体遗传特征
Unfolding of Genetic Characterization of Binglangjiang Buffalo by Microsatellite DNA Markers
摘要
Abstract
Binglangjiang buffalo is the first indigenous river type buffalo which has been found in western Yunnan province of China in recent years. In order to elucidate genetic background information on its genetic diversity, genetic composition, genetic relationship with typical river and swamp buffalo, and the gene introgression from swamp buffalo, 141 random samples from Binglangjiang buffalo population and 24 samples from control river buffalo population (Murrah) and 41 samples from control swamp buffalo population (Diandongnan buffalo) were genotyped by employing 30 microsatellite DNA markers. The result showed that a total of 253 alleles were identified in Binglangjiang buffalo (including 54 alleles shared among the three populations assayed, 58 alleles only shared between Binglangjiang and Murrah buffalo, 73 alleles only shared between Binglangjiang and Diandongnan buffalo, and 68 alleles unique to Binglangjiang buffalo). The genetic diversity parameters in Binglangjiang buffalo were much higher than that in the control groups, its mean number of alleles per locus, mean observed heterozygosity, mean expected heterozygosity and polymorphism information content (PIC) were 8. 433 3, 0. 640 5, 0. 600 9 and 0. 594 7, respectively. Inbreeding coefficient (Fis) in Binglangjiang buffalo was 0. 061 9. Genetic distance(DA) between Binglangjiang and Murrah buffalo was the lowest(0.114 4), while genetic distance(DA) between Diandongnan and Binglangjian buffalo(0. 382 9) or between Diandongnan and Murrah buffalo(0. 555 3) was much larger than that between the two formers, which make Binglangjiang buffalo and Murrah buffalo, grouped together and Diandongnan independently clustered in the NJ tree. Structure analysis revealed that Binglangjiang buffalo displayed a genetic mixed pattern by river buffalo and swamp buffalo, most of the individuals having some proportion of swamp buffalo component (membership coefficients =0. 067 2). The results indicate that Binglangjiang buffalo is of high degree of genetic diversity and is unique genetic resource, but this buffalo is under the conditions of heterozygote deficit and genetic mixture with swamp buffalo.关键词
槟榔江水牛/微卫星DNA标记/遗传变异/基因渗入Key words
Binglangjiang buffalo/ microsatellite DNA marker/ genetic variation/ gene introgres-sion分类
农业科技引用本文复制引用
刘伟,苗永旺,李大林,唐贺,张慧芳,章纯熙..利用微卫星DNA标记分析槟榔江水牛群体遗传特征[J].畜牧兽医学报,2011,42(11):1543-1549,7.基金项目
云南省应用基础研究重点项目(2007C0003Z) （2007C0003Z）
国家自然科学基金项目(30660024) （30660024）
云南省应用基础研究计划面上项目(2006C0034M) （2006C0034M）
国家高技术研究发展计划(863计划)项目(2008AA101001) （863计划）
