畜牧兽医学报2011，Vol.42Issue(11)：1577-1583,7.
鸡传染性法氏囊病病毒VP2基因突变对病毒体外复制的影响
Influence of the Mutations of VP2 on the Replication Efficiency of Infectious Bursal Disease Virus in vitro
摘要
Abstract
To study the molecular basis for the replication efficiency and virulence of infectious bursal disease virus (IBDV), two amino acid mutations (A222P, D279N) was introduced in to VP2 of IBDV, respectively, based on the information of sequence analysis. Using the reverse genetic research system, two responding mutated strains of IBDV were rescued and their biological characteristics were studied. The replication kinetic curves showed that the rescued virus including A222P replicated over 6 times higher than its parent strain did, while D279N couldn't. Ani- mal experiment of SPF chickens showed that residue 222 and 279 of VP2 did not contribute to virulence of IBDV. The relations between the two residues of VP2 (222, 279) and the replication and virulence of IBDV were reported firstly. The findings provide necessary information for further understanding the gene function of IBDV and the design of new tailored IBDV vaccines.关键词
鸡传染性法氏囊病病毒/VP2基因/复制/致病力/反向遗传Key words
infectious bursal disease virus (IBDV)/ VP2 gene/ replication/ virulence/reverse genetics分类
农业科技引用本文复制引用
祁小乐,高宏雷,刘娣,华育平,王笑梅,高立,吴关,邓小芸,余飞,张礼洲,秦立廷,高玉龙,王永强..鸡传染性法氏囊病病毒VP2基因突变对病毒体外复制的影响[J].畜牧兽医学报,2011,42(11):1577-1583,7.基金项目
国家973项目(2005CB523202) （2005CB523202）
国家自然科学基金(30901083) （30901083）
中国博士后科学基金(20080440921) （20080440921）
黑龙江省博士后基金(LBH-Z08016) （LBH-Z08016）
