畜牧兽医学报2011，Vol.42Issue(11)：1591-1597,7.
猪嗜血支原体酶标单抗阻断ELISA检测方法的建立
Development of Blocking ELISA for Mycoplasma suis with Peroxidase-Labelled Monoclonal Antibody Against MSG1 Protein
摘要
Abstract
To provide a serological method for detecting antibodies against Mycoplasma suis (M. Suis), a blocking ELISA was developed based on a monoclonal antibody (Mab) to M. Suis-VMSGl protein. The conditions for each step were optimized. The optimal concentration of the coating antigen was 0. 5 (μg · mL-1 ; sera samples diluted 1 fold and incubated 1. 5 h at 37 ℃; Mab-HRP dilution is 10 000 fold and incubated 1 h at 37 ℃. The blocking results of one hundred M. Suis negative sera samples were statistically analyzed, and the cutoff of blocking ELISA was determined that the samples presenting a percentage inhibition of ≥ 23. 71% were considered positive; samples with a calculated percentage inhibition of ≤ 36. 35% were rated negative and those presenting a blocking effect between 23. 71% and 36. 35% were considered inconclusive. The Blocking ELISA proved to be specific, sensitive and it showed high reproducibility and low variability. This method will be useful in clinical detection and epidemiological study on M. Suis.关键词
猪嗜血支原体/MSG1蛋白/酶标单抗/阻断ELISAKey words
Mycoplasma suis % MSG1 protein/ monoclonal antibody/ blocking ELISA分类
农业科技引用本文复制引用
张长莹,李玉峰,姜平..猪嗜血支原体酶标单抗阻断ELISA检测方法的建立[J].畜牧兽医学报,2011,42(11):1591-1597,7.基金项目
公益性行业(农业)科研专项项目(200903036-10) （农业）
